In this thesis , the energy transfer system of acridine orange ( ao ) - rhodamine b ( rb ) dimer was built for the determination of dna as fluorescence probe 本论文以吖啶橙?罗丹明b二聚体能量转移体系作为荧光探针，建立了测定dna的新方法。
Some new fluorescent probes and sensors are prepared using these techniques , and they are utilized to determine some medicines and toxic materials in the environments 利用这些方法制备了几种新型荧光探针及传感器,并用于药物和环境毒物的检测中。
Two new fluorescent ca2 + indicators stdin and stdbt have been designed and synthesized . the structures and absorption and fluorescence spectral characteristics of these reagents have been thoroughly studied 2 .新型钙荧光探针的性能研究研究了试剂的结构、吸收及荧光光谱特性和细胞荧光标记特性。
For these studies , they developed laboratory mice engineered with fluorescent probes in their immune system that light up when mucosal barriers , such as the lining of the intestines or lung , come under attack 为了这些研究，他们发展了在免疫系统中带有荧光探针的实验用小鼠，当粘膜屏障，例如肠道和肺的内层，遭受攻击时发光。
Dna content , intracellular ca2 + and mitochondrial membrane potential in cultured cbt or cp were analyzed . 02 ~ content was useing nbt method , activity of sod was determined based on auto - oxidation of pyrogallol 用荧光探针pi (碘化丙碇) 、 indo - 1及rhodamin123分别检测细胞在低温度处理后dna含量、细胞内钙离子浓度和线粒体膜电位。
Fluo - 3 , a visible light - excitable ca2 + indicator , overcomes the limitation of quin - 2 , indo - 1 and fura - 2 but no longer display any spectral shifts upon ca2 + binding and thus rati ometric measurements are not possible 表明所合成试剂stdin ? am是目前国内外唯一的可以am酯型无损伤导入细胞、双波长、可见光激发的胞浆特异性ca ~ ( 2 + )荧光探针。
Laser scanning confocal microscope combined with fluorescence probe and fluorescence resonance energy transfer techniques has become a very effective tool of researching the behavior of massive molecules in living cells 摘要激光扫描共聚焦显微镜结合荧光探针以及荧光共振能量转移技术，已成为近年来应用在活细胞中研究大分子行为的一种非常有效的研究工具。
The quantitative determination of protein and dna is a common analysis contents in biology chemistry and life science . so it is very important to find fluorescence probe with high sensitivity and low detection limit 对蛋白质和dna进行定量测定是生物化学和生命科学中经常涉及的分析内容，建立高灵敏度、低检测限的荧光探针定量测定的方法仍具有重要意义。
H2o2 generation in guard cells was examined by laser scanning confocal microscopy based on fluorescence probe h2dcfda . the fluorescence intensity in guard cells of wild type and ios5 was essentially the same before treatment 以h2o2荧光探针h2dcfda结合激光扫描共聚焦显微技术直接检测了保卫细胞内的h2o2产生情况。未加任何处理之前，野生型与los5保卫细胞内荧光强度几乎相等。
It is interesting to find that the green - emission band in solvent solution has been quenched , meanwhile , the blue - emission band appeared at the present of b - cd , suggesting that molecular materials have sensitive to the micro - circumstance and have potential application for fluorescence probe 这种由极性环境到非极性环境引起的发射光峰位明显变化特性，即对微环境的敏感性，具有作为荧光探针的潜在性能。
