The main research contents include the methods for trehalose determination , the breeding of trehalose high - producing strain , the optimization of medium and culture conditions , the production of extracellular trehalose and the purification of the intra - and extracellular trehalose 研究内容主要包括海藻糖测定方法的研究、高产海藻糖菌株的选育、培养基与培养条件的优化、胞外海藻糖的合成以及海藻糖的提取与精制等。
Meanwhile , the results showed that althougt the trehalose was produced successively , the intracellular trehalose concentration was 0 at the end of the reaction . trehalose was excreted to culture medium completely . yeast ' s cell was only as a carrier of the trehalose synthase 利用酵母海藻糖合成酶系合成胞外海藻糖是海藻糖生产方法的一个创新，它结合了酶法和酵母提取法的优点，是利用酵母大量生产海藻糖的又一新的尝试。
Comparing with the y7 , the specific growth rate of mutant increased by 22 . 7 % , the activities of neutral and acid trehalase droped by 19 . 2 % and 6 . 7 % , respectively . under the non - optimized condition , the production of trehalose increased by 57 . 2 % compared with that of parental strain y7 与原菌株y7相比较，对数期比生长速率提高22 . 7 ，酸性海藻糖分解酶活性下降19 . 2 ，中性海藻糖分解酶活性下降26 . 7 ，胞内海藻糖含量提高了57 . 2 。
