The protein level of p21 increased lightly from 1 to3 h , then decreased at 6 - 9 h , reached maximum at 24 h after 2gy , it increased from 3 to 24 h exposed tology , and the protein level of p21 associated with cdk2 increased , which did not inhibit the cyclin e / cdk2 kinase activity , because of upregulation of cyclin e and deregulation of p27kipl after irradiation . in contrast to p21 , p27kipl may be a key regulator of cyclin e / cdk2 activity in dna damage responses . hi the ubiquitin - proteasome pathway invovled in regulation of p27kiplexpression in response to ir the ubiquitin - proteasome pathway regulated selective and time - controlled elimination of p27 kipl 与p27ki ” ，表达水平不同， pzi蛋白表达水平在zgy照射后略有增加， 6一gh略有下降，在照后24h达到最大; 10gy照后1一3h开始增加，持续到24h ，并且cycline / cdkz复合物中pzi蛋白结合增多，但cycline / cdkz激酶活性并未受到抑制，除了因ir诱导cydine蛋白表达水平增加外，另一重要的原因就是与pzi同一家族的p27kip ，蛋白表达水平下降，失去了对cycline / cdkz的抑制活性。
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei , and to study the function and mechanism of mint - mediated transcription repression , yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5 , amino acids 2226 - 2959 ) . from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo , fifty - one were positive for nutritional screening and p - galactosidase assay . restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments , which are mint , alpha a - crystallin - binding protein i ( alphaa - crybpl ) , and nuclear receptor co - repressor 1 ( n - corl ) , respectively 鉴于mwt基因编码区较长，共有10799个碱基，故此我们将mint分为六段，分别命名为fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段为研究对象，将h者分别插入pgb盯7载体中，结果显示： mintfs可与核受体辅助抑制因子1阶cori ） ， o晶状体蛋白结合蛋白1hlphatcrybp入及mw加互作用，而f6可与igm的重链恒定区、泛素结合酶2l6和一个未知功能的新的蛋白基因进行结合。
The scfco , " ubiquitin - ligase complexes are required for jasmonate response in arabidopsis ( l ) arabidopsis coi is required for all the jasmonate - regulated response . it encodes a protein containing leucine - rich repeats and a degenerate f - box motif these structural features are characteristic of f - box proteins that function in ubiquitin ligase complexes for the ubiquitylation of substrate - proteins targeted for degradat1on 以表达融合蛋白flag - coi1的拟南芥为材料，用- flag抗体进行免疫共沉淀分析发现， coi1蛋白可以与ask1 、 ask2 、 atrbx1和atcul1蛋白结合形成scf ~ ( coi1 )复合体，并发现其中的atcul1蛋白包括未修饰和修饰的两种形式。
Otherwise ; we have used immunoprecipitation and im - munofluores cence microscope technology to study the interaction with doc - 1r and cdk2 proteins under physiological condition in cells , we attempt to find the evidence of interaction with doc - 1r and cdk2 proteins , which will offer experiment basis for understanding the accurate role for doc - 1r gene in cell cycle s regulation 另外我们采用免疫共沉淀技术和免疫荧光显微技术观察在生理条件下细胞内doc - 1r和cdk2蛋白间相互作用的情况，试图找到细胞内doc - 1r与cdk2蛋白结合的证据，为准确掌握doc - 1r基因在细胞周期调控中的作用提供实验依据。
