Dissecting the prothoracic glands , by using silkworms " prothoracic glands in vitro culture and h - ecdysteroid - radioimmunoassay , the biological activities of those analog peptides were examined , and their active features , chemical parameters , as well as the relationship between structures and functions of those peptides were analyzed and compared . with an exception of 899808 having the sequence of awqdlsaw that affected like bom - ptsp , 899805 ( awqdlns ) and 899809 ( awqdlnsgaw ) partially stimulated ecdysteroidogenesis in a dose dependent manner , whereas 899803 ( dlnsaw ) , 899804 ( awqdlnsa ) , 899806 ( awqdln ) as well as 899807 ( awqdlnsawa ) had both stimulatory and inhibitory effects on ecdysteroidogenesis , depending on the concentrations 研究结果表明，类似多肽89980吕（ awq uawaw ）的生物学功能与抑前胸腺肽的相同而且活性近似：类似多肽899805 （ awqdlns ） 899809 （ awqdlns … ）对家蚕前胸腺蜕皮激素的生物合成表现出随浓度增加而增加的促进作用，而低浓度下几乎不促进； 899803 （ dlnsawawl899804 （ awqdmsa ） 、 899806 （ awqdln ）和899807 （ awqdlnsawa ）类似多肽对家蚕前胸腺蜕皮激素的生物合成的促进和抑制作用与它们的浓度有着依赖关系。
Techniques of culture of prothoracic gland ( pg ) in vitro , 3h - ecdysteroid - radiounniunoassay , and methods of molecular biology and biophysics , for instance , nuclear magnetic resonance ( nmr ) , circular dichroism cd ) and so on were engaged . the biological activity on ecdysteroidogenesis in vitro and in vivo of a group of analog peptides of bom - ptsp synthesized by chemical modifications and the relationship between the structures and functions were studied 本研究利用~ 3h -蜕皮激素放射免疫分析方法、分子生物学、生物物理学的方法和手段、体外培养和体内导入等技术对bom - ptsp及其一组人工合成的与ptsp结构类似的多肽进行了结构和功能关系的研究，鉴定了它们的生理活性及其与结构的关系，以及它们在家蚕体内的活性及其与结构的关系，还对ptsp及其类似多肽进行了核磁共振和圆二色的研究。
The main function of bom - ptsp is that it inhibits ecdysteroidogenesis when prothoracic gland ( pg ) of the silkworm was active , and when pg is not active , prothoracicotropic hormone ( ptth ) activate pg , whereas bom - ptsp almost completely inhibits ptth - stimulated ecdysteroidogenesis when pg was incubated in medium of ptth and bom - ptsp combination in vitro . ptsp cooperates with ptth to regulate and control ecdysteroidogenesis on prothoracic gland of insect 它的主要作用是：当家蚕前胸腺( prothoracicgland , pg )处于活动时期，该多肽能强列地抑制前胸腺合成蜕皮激素；当前胸腺处于非活动时期，促前胸腺素( prothoracicotropichormone ， ptth )可以激活前胸腺；同时用ptth和ptsp体外培养前胸腺时，被ptth激活的蜕皮激素的生物合成又可被ptsp所抑制。
