贝可

• 5 . the position of buffalo oocyte nucleolus related to its pb1 during the final stage of ivm , and the feasibility of increasing enucleation rate using spindle - view system were studied . the space between pbi and spindle ( sp ) of ivm buffalo oocytes increased as the ivm duration prolonged
  9 ， 64刀） ，但当成熟时间增加到24h时，这一比例下降到57石（ 726 vs57石， p 0刀5 ） ，如采用spindle view系统去核贝可消除这种位置变化对去核率的影响。