Protection against synergistic hepatocarcinogenesis of hepatitis b virus expression and aflatoxin b1 by antioxidant 2 - tert - 4 - hydroxyanisole in hbv large envelope transgenic mice 抗氧化剂对黄曲霉毒素染毒的转基因小鼠肝肿瘤的防护
One approach is to find tumour - associated and microenvironment - associated oncoantigens required for progression from one tumour stage to the next by comparing gene signatures isolated from the different stages of tumour progression in cancer - prone transgenic mice 一种方法就是去寻找肿瘤相关及微环境相关的癌基因，而这些癌基因则是通过比较患瘤倾向的转基因小鼠的肿瘤进展不同阶段分离出来的基因信号而来发现从肿瘤发展的一个阶段到下一个阶段所必需的基因。
Secondly , the chicken r - ifn cdna was introduced into the vector pbcas between the bovine asi - casein 5 ' regulation region and 3 ' flanking region to create a vector pbcasifn to examine if this bovine asi - casein 5 ' regulation region and 3 ' flanking region could regulate chicken y - ifn to express in the latex by producting transgenic mice 最后，我们将鸡?干扰素cdna插入到载体pbcas的牛s1 ?酪蛋白的5 ’调控区和3 ’侧翼区之间，构建成载体pbcasifn ，以便于通过生产转基因小鼠来检验牛s1 ?酪蛋白的5 ’调控区和3 ’侧翼区是否可以调控外源基因鸡?干扰素在乳腺中特异表达。
On the other hand , - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established . methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation . this vector is used to pronucleus microinjection 本实验以pegfp - n1质粒为骨架载体，用酶切连接的方法构建一个顺序含有- actin启动子、 fad2cdna 、 sv40polya加尾信号的真核表达载体，双切线性化后回收，使用回收的表达载体经原核显微注射生产转基因小鼠。
In contrast , transgenic mice overexpressing gat1 displayed ethanol tolerance , as shown by the righting reflex test . mi ce overexpressing gat1 survived a lethal dose of ethanol ( 9g / kg , i . p . ) longer , maintained locomotor activity longer after a sub - lethal dose ( 1 . 75g / kg , i . p . ) 相反，在翻正实验中过量表达gat1的转基因小鼠表现出显著的酒精耐受；与同胎所生的野生型小鼠相比，转基因小鼠在酒精致死剂量（ 9g / kg ， i . p . ）下存活较长时间；在酒精低剂量（ 1 . 75g / kg ， i . p . ）下维持更长时间的运动活力，相应的ld50也较高。
