Ctla4ig fusion protein was expressed by genetically fusing the dna fragment encoding 1 - 125 amino acid residues of ctla4 extracellular domain with dna fragment encoding an igg fc region . its in functional region is the extracellular domain of ctla4 Ctla4ig融合蛋白是通过基因重组技术将编码ctla4胞外区1 - 125个氨基酸序列的dna片段与编码iggfc段的dna片段融合后表达生成，其功能区是ctla4胞外区。
Symposium participants reaffirmed that agricultural biotechnology will lessen the demand of agricultural products worldwide while protecting the environment and natural resources . the public concerns on recombinant dna technology and genetically modified food were also discussed 在研讨会上，讲者均重申农业生物技术对舒缓全球农产品需求及保护环境与天然资源的重要性，并讨论了社会上对基因重组技术及基因食品的关注。
Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress 最近的研究表明，基因转移与重组技术在结缕草遗传改良中具有巨大应用潜力；结缕草遗传连锁图谱构建取得重要进展；基因克隆和基因资源研究正在展开。
In the research of transgenic fish , green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology . thus pagfp plasmid was constructed successfully . the recombination was determined by digestion of restriction enzyme and sequencing 实验通过分子重组技术，采用定向克隆法将绿色荧光蛋白基因亚克隆到puc118a上鲤鱼-肌动蛋白基因启动子下游，构建成能在真核生物体内表达的表达载体pagfp ，经双酶酶切法序列鉴定后，回收带启动子和目的基因片段。
This problem - solving process should include : taking the informationization as a forerunner and develop the flatness of tax affairs management ; establishing the smooth information management system and developing the application room for information increment : enhance the scientific level of management and decision - making based on the data analysis ; rearranging the technology resources to realize the informationization of tax affair , enforce the tax management of electronic business activities 文章最后综合上面的论述，提出了我国税务信息化的发展应该有针对性的集中解决一些突出问题，包括以信息化为先导，促进税务管理扁平化；建立流程管理信息系统，拓展信息增值应用空间；以数据处理分析为支点提升管理决策科学化水平；重组技术资源，实现税收信息化；加强对电子商务的税收管理。
These results suggested that the 65kda protein was a degraded product , bioassay result showed that insecticidal activity of bl - 3 was 3 - fold higher than that of bl - 2 against 3rd instar plutelh xylostella . 3 . expression of cryla ( b ) gene in pseudom on a s fluoreseent pfx - 18 in order to study expression characteristics of icps gene in pseudom onas fluorescent , a new simple transformation method of pf was established 3 .用上述重组技术获得了含有刀口基因的lz20和含有尸2拼即“的lz20a克隆株，通过基因的诱导表达，发现p ”和pz浏民有不同的作用机制，证明了p20具有保护cyt人溶细胞的作用，这种作用并不依赖大肠杆菌受休的不同而改变
In the detection of protocol analysis model , we mainly studied the fragment reassembly of ip packet on the bases study of tcp / ip protocol . in the detection of pattern match model , we analyzed several kinds of pattern match algorithms such as : kmp , bm , bmh and multiple pattern match algorithms ( wang 2002 ) 在基于协议分析的检测方法中，从tcp / ip协议族的层次结构出发，主要分析了ip包的分片重组技术；在基于模式匹配的检测方法中，主要分析了多种模式匹配算法： kmp 、 bm 、 bm的改进算法以及多模式匹配算法。
For example , in 20 centuries 70 time , as the progress of molecular biology , be united in wedlock with project technology photograph , open up biology technology new field : gene recombines technology of cross of technology , pcr technology , dna and technology of protein alignment analysis , element to wait , the birth that promoted gene project , protein project , cell project , enzymatic project to wait and development 例如，在20世纪70年代，随着分子生物学的进步，与工程技术相结合，开辟了生物技术新领域：基因重组技术、 pcr技术、 dna和蛋白质序列分析技术、分子杂交技术等，促进了基因工程、蛋白质工程、细胞工程、酶工程等的诞生和发展。
However , its wide application in manufacture has always been restricted by such a question as low phytase - producing level of wild strains , so before the wild strains which can produce acidic phytase are widely used , their phytase activities must be improved by various means , in which using an efficient expression system to express heterogenous phytase is a main consideration 植酸酶主要存在于微生物和植物中，动物体内也有少量存在，但真正具有开发价值的主要是微生物产生的植酸酶，尤其是曲霉产生的酸性植酸酶。国外早在1991年就成功地利用dna重组技术获得第一株酸性植酸酶的工程菌，并已投入商品化生产。
Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting 目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。
