Labeling tunel method . the cell ultrastructural changes were similar to apoptosis in animal cells : the apical meristemetic cells underwent the programmed cell death . this was first detected in the apex cells of apical meristem , while peripheral cells differentiated gradually into different parts of a floral bud . but all the cells in the floral bud were subjected to the pcd process before it developed into a complete flower . 140bp dna fragment was found to deposit in apical bud during the plant development . the most important role of caspase - 8 was detected by western blot , and the expression of the procaspase - 8 was time - related with the dna frgmentation and the transformation from vegetative to the reproductive growth . these results suggested that pcd was an active process during the differentiation of apical meristem , and the senescence observed in the apical bud was due to the pcd process 显微超微结构研究表明，短日照条件下豌豆顶芽的衰老过程是从营养生长锥向花芽的转化，而用dna原位末端标记tunel caspase - 8 western blot和140 bp dna片断积累的试验结果证明，转化为花芽的整个生长锥细胞发生了编程性死亡pcd ，而且其最顶端部分细胞首先发生pcd ，而顶端周围的分生组织细胞逐渐分化出花芽的各部分，但顶芽最后并没有发育成为完整的花，所有细胞就都发生pcd ，从而顶芽衰老。
The process of initial culture showed quite difference in various hostas . h . ventricosa and h . " so sweet " were easier , while h . " frances williams " and h . " gold standard " were difficult . using shoot tip ( peak bud ) as explant was proved the effective method for improving survival ratio when hostas would defoliate or have defoliated in autumn or winter rather than in summer 玉簪属不同种类的组培初代建立存在着一定的差异，如h . ventricosa和h . ‘ sosweet ’较容易建立，而h . ‘ franceswilliams ’和h . ‘ goldstandard ’比较困难；应在冬季玉簪属植物将要落叶或已落叶后取茎尖(顶芽)进行初代建立，最好避开夏季，来提高初代建立的成活率。
