calf serum造句

• The concentration of fbs ( fetal bovine serum ) and ncs ( newborn calf serum ) was influential on the culture process of mef ( mouse embryonic fibroblast ) and es cells
  血清浓度的高低对胎鼠成纤维细胞和es细胞的培养过程是有影响的。无血清或低浓度的血清能抑制细胞分裂增殖，使细胞生长出现停滞。
• Various concentrations of rrta and rrta - yqrl were added into the wells and incubated for 6 h to allow internalization of toxins by fluid - phase endocytosis . the cells were then incubated in rpmi - 1640 medium containing 10 % fetal calf serum for another 24 h
  结论本实验用基因工程方法原核表达了rl ’ a蛋白和rl ’ a一yqrl蛋白，利用rta蛋白和f3ga的结合特性，温和、简单、有效地洗脱了目的蛋白。
• But in experiment ii , the obtained result of measurement and examination is not better than control group 。 conclusion : mixed animo acids can be safely applyed to vessel cryopreservation instead of calf serum 。 the effect of the groups of the rapid freezing method is not better than the groups of the rate - controled slow freezing
  而实验ii的血管其内皮结构不清晰，内皮细胞超微结构变化较大。结论：复方氨基酸可代替胎牛血清用于血管的深低温保存.速冻降温方式对血管深低温保存效果不及慢冻降温方式。
• Method : 120 rabbit vessels were randomly divided into three groups : the vessel of control group in cryopreservative medium containing calf serum were subjeted to rate - controled slow freezing ; those of experiment group i in cryopreservative medium containing mixed animo acids were frozen by the same method as in control group ; and those of experiment group ii in the same cryopreserative medium as in control group were subjected to rapid freezing
  材料和方法：取中国纯种家兔股动脉120段，随机分为3组：对照组用含胎牛血清的保护液处理，以慢冻方式降温；实验组1用含复方氨基酸的保护液处理，以慢冻方式降温；实验组ii用含胎牛血清的保护液处理，用速冻方式降温。
• In order to study the function of cycling2 in vitro culturing cell line , we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent , and studied the function of cycling2 expression on the cell proliferation in vitro , further investigated the regulation mechanism of cycling2 . at the same time , we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue . material and method 1 material : piresneo vector was purchased from clonetech , plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640 , , dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit , mouse monoclonal antibody p21wafl ( in use ) , dab staining kit were purchased from maixin company
  实验材料与方法1 .实验试剂高糖dmem 、 rpmll640和胎牛血清购自美国g山eo / brl公司; dmewf12 ( 1 : 1 )混合培养液购自美国hyclone公司;胰蛋白酶购自美国si目叮a公司; hepes由美国amersco公司分装;脂质体转染试剂( upofectalnineplusreageni )和以18为美国玩vitrogen公司产品; piresneo载体购自美国cloneteeh公司;质粒提取及纯化试剂盒购自德国qiagen公司; ultresensitive翎s一p免疫组织化学试剂盒;鼠单克隆抗体户3 ( do一7 )蛋白(即用型) ;鼠单克隆抗体p21waf ， (即用型) ; dab染色试剂盒均购自福建迈新公司;鼠单克隆抗体pziwa曰(浓缩型) ;辣根过氧化酶标记羊抗鼠二抗购自北京中山公司; ecl试剂盒购自美国santacruze公司; dcproteinassay试剂盒购自bi 。