pcr amplification造句

• Research of pcr amplification factor from tea gene
  扩增条件研究
• Genetic variations of storage protein genes among chinese endemic wheats detected by pcr amplification
  型高分子量谷蛋白亚基基因的多态性
• 3 . establishing contamination free pcr amplification system by adding ung enzyme . 4
  于反应体系中引入ung去污染酶，建立了无污染荧光pcr扩增体系； 3
• 6 . in this study , establishing contamination free pcr amplification system by adding ung enzyme
  6 、于反应体系中引入ung酶，建立了无污染的荧光pcr扩增体系。
• The pcr amplification products were analyzed by vertical denaturing polyacrylamide gel electrophoresis followed by silver staining
  Ys393二基因座， pag电泳分型，银染显色。
• The transformation of hairy roots was confirmed by pcr amplification of rolb and rolc genes of ri plasmid from a . rhizogenes
  Pcr扩增结果证实， ri质粒的t - dna部分已在红龙草毛状根的基因组中整合及表达。
• Results 1 pcr amplification result 18 among 48 isolates were amplified successfully and positive rate was 37 . 5 %
  实验结果一jcr扩增结果对48株低传代临床分离株进行hcmvul136全序列pcr扩增，结果18株阳性，阳性率37
• Pcr amplification results indicated that aii gene could be found in b . thuringiensis , b . cereus , b . subtilis and b . sphaericus strains , but not in b . megaterium
  在苏云金芽胞杆菌不同亚种中均可检测到aii蛋白活性和aii基因。
• The cdna probe was prepared by pcr amplification technique . colorimetric and chemiluminescent detection for biotinylated probe were used in this test
  同时，利用双向电泳和rt - pcr方法进行类病毒鉴定，证明两种方法均可用来检测类病毒。
• Power for the thermal cycler shall be provided with a manostat or a ups to avoid the influence on pcr amplification arose by fluctuation of voltage
  热循环仪的电源应专用，并配备一个稳压电源或ups ，以防止由于电压的波动对扩增测定的影响。
• The pcr amplification products were separated on 6 % vertical denaturing polyacrylamide gels . numerous and distinct bands could be detected by silver staining
  Pcr扩增产物经6变性聚丙烯酰胺凝胶垂直电泳分离后，银染能检测到多而清晰的条带。
• The aflp ( amplified fragment length polymorphism ) technique is based on the selective pcr amplification of restriction fragments from genomic dna digested by restriction enzymes completely
  扩增片段长度多态性技术( aflp )是基于选择性扩增完全酶切消化后的基因组dna片段。
• Confirmation of transformant . integration of sag - ipt into chrysanthemum genome was confirmed in two thirds of the putative tansgenic plantlet be pcr amplification
  通过pcr扩增表明，转基因植株中可以检测出有目的基因( ipt基因)的存在，目的基因已成功地插入菊花的基因组中。
• Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed . 3
  通过合成六条长链引物，经两次pcr扩增，获得了238bp的植物防御素afp基因全长，并构建了植物表达载体peafp 。
• Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system
  进一步通过rt . pcr克隆了ked样蛋白编码区全长cdna ，通过双杂交验证了全长ked样蛋白与acamz的相互作用，结果为阳性。
• By means of pcr amplification to total dna extracted from four transgenic plants , it indicated that bet - a gene had been integrated into the haploid of the hybrid genome
  最终得到四个转化株系，经pcr检测，全部产生特异性扩增谱带，证明bet - a基因已整合进小黑杨单倍体植株的基因组中。
• With the improved method of ctab , the genomic dna extracted form plum and other species close to p . mume in genetic relationship could be directly used in pcr amplification , with good results obtained
  选用改良ctab法提取的梅及其近缘种的基因组dna可直接用于pcr扩增分析，且效果良好。
• We cloned full - length cdna of ca coding region via pcr amplification , but a negative result was obtained when the interaction of full - length ca and ga was tested in yeast two - hybrid system
  通过rt - pcr克隆了ca编码区全长cdna ，但是在双杂交系统检测全长ca与g蛋白的相互作用时，结果为阴性。
• E2 gene was cut with restriction endonuclease from the recombination plasmid t - e2 , and subcloned into the expression vector ppic9 ' s mcs , which was identified by enzyme digestion and pcr amplification
  将重组质粒t - e2的e2基因酶切后，亚克隆到表达载体ppic9上，经酶切和pcr鉴定，命名为ppic9 - e2 。
• It also was a cosmopolitan species in the northern hemisphere . the phylogenetics relationships between the isolates of a . gallica from europe , north america and china were studied with issr - pcr amplification
  并对这5个蜜环菌生物种的地理分布、生长规律、寄主范围、培养特性等生物学习性进行了研究讨论。