pcr analysis造句

• Studies on cultivar classification of osmanthus fragrans by issr - pcr analysis
  技术在桂花品种分类研究中的应用
• After that we determined the presence of angiostatin gene in the putative recombinant virus with pcr analysis
  之后利用pcr分析是否获得重组angiostatin的杆状病毒。
• 2 . 3 gene expression identification of neural related genes by semi - quanti - tive rt - pcr analysis
  3半定量rt - pcr在mrna水平检测escs 、神经干细胞及神经细胞相关基因。
• Rt / pcr analysis showed that ams gene expresses in leaves , stems and flowers . the expression was n ' t detected in roots
  Rt pcr分析表明ams基因在叶片、茎和花中表达，而在根中没有表达。
• Rt - pcr analysis was used to determine the levels of grp78 and grp94 mrna during the different phase of mouse development 2
  Rt - pcr方法检测发育不同时期小鼠胚胎脑组织中grp78 、 grp94mrna表达情况2
• Pcr analysis and southern blotting all confirmed that the ced - 9 gene has been integrated into the chromosomal dna of these transgenic plants
  对所得到96株再生苗进行pcr检测，结果表明，阳性苗比例为34 。
• Given limitation existing in all those methods , pcr analysis , an efficient method for rapid screening and diagnosis , has been used more and more
  因此，目前作为快速筛查和诊断的另一种有效方法? ? pcr分析方法? ?正在越来越多地被应用。
• Pcr analysis showed rip gene has integrated into rice genome primarily . the positive tran - sgentic plants with lba4404 ( p3301rjp ) is dongnongv - 10 2 and fushiguang 2
  Lbamp330lrip ）转化水稻的pcr检测阳性株数为：东农v102株，富士光2株。
• Purple clones were picked out from the plate , which show br was expressed . pcr analysis told us that the mutant br gene was transformed into l33
  Br基因的定点突变改造和突变基因在嗜盐菌中表达系统的建立使我们可以研究br的各种突变蛋白。
• Gus examinaton showed that the rate of transgene was higher . and pcr analysis , pcr - southem and southern analysis all showed that chs was intigrated into quamocliy pennata chromosome successfully
  同时，还以dig标记的camv35s上的一段为探针进行southern点杂交和pcr - southern杂交。
• Pcr analysis of resistant seedlings with nptii gene primers showed that 6 out of 12 seedlings detected had the 700bp fragment specific to the plasmid pig121 , indicating that t - dna had been integrated into the genome of sweet cherry
  L ，负压的适宜处理是lmmxio次。 6 、通过p r扩增，初步证明证明外源基回己转入甜樱桃。
• Restriction enzyme digestion analysis and pcr analysis showed that these two plant expression vectors constructed were correct . 49caixin , one cultivar of the brassica campestris l . ssp . chinensis , was transformed by vacuum infiltration method
  通过真空渗入和花序浸渍法，将上述双抗和单抗基因分别导入白菜不结球类型? ? 49菜心中，获得了7棵抗性株。
• Northern blot and rt - pcr analysis show that sh2a gene is ubiquitously expressed in many tissues with three transcripts . the aberrant expression of sh2a gene in some cancers was found . it suggests that sh2a gene relates to tumors
  Rtpcr及northern印迹杂交发现sh人在多种组织中有表?二?达，有3个转录本，而且在癌及癌旁组织的比较中发现，肿瘤组织中表达较高，初步证明与肿瘤的发生有关。
• As sampling of bioaerosols in natural environments is likely to be associated with substantial contamination by a range of microorganisms commonly existing in an ambient air , an investigation of the specificity of detection by targeted pcr analysis is required
  于真实环境中采样生物性气胶往往会被一般大气中常存之优势微生物所污染，故需针对特定微生物发展其特定聚合酵素反应技术相关条件。
• Pcr analysis showed that approximate 9 % tested plants produced the target band . a few of the 9 % plants produced the band in southern blot analysis . additionally , the number of inserted copies of dsg 10 was different in different transgenic plants
  结果显示有9的再生苗为pcr阳性，对pcr检测阳性苗进行southem杂交分析，发现dsg10已整合入烟草的基因组中，而且在这些苗中dsg10片段整合的拷贝数不同，部分pcr阳性苗southern杂交结果却是阴性。
• There are four imperfect repeats v - v - e - k - k - n / e - e of which the core sequence is similar to map ib of mouse . rna blot and rt - pcr analysis showed that this gene is expressed specifically in the mature pollen and can be classified as a late gene in pollen development
  计算机软件分析st901基因核苷酸、氨基酸序列的相似性，结果表明， st901基因编码区与探针sb401 、与高赖氨酸基因sblr 、与番茄tsb具有较高的相似性，它们可能来源于马铃薯的一个基因家族。
• Northern blot and rt - pcr analysis verified that the expression level of ldplcl was induced in germinating pollen , whereas ldplc2 expression level did not change during pollen germinating process . the interaction between heterotrimeric g proteins and plc was detected in a yeast two - hybrid system
  Northern和rt - pcr分析，发现随着花粉的萌发， ldplc2的转录活性提高，而ldplc1博士学位论文在萌发前后的转录水平没有变化，推测至少ldplcz参与了对花粉萌发的调控。
• Pcr analysis indicated that all lines had been integrated of ssmapkk . northern analysis revealed the presence of expression of ssmapkk mrna in transgenic lines . in principle , ssvp overexpression can increase proton electrochemical gradients across the vacuolar membranes , which permit the secondary active transport of na + and solute molecules
  理论上， ssop的过量表达可增加转基因植株细胞跨液泡膜的质子电化学梯度，为次级转运提供驱动力，从而增加可溶性物质和na十向液泡内的转运，提高转基因植株的抗旱和抗盐性。
• 4 . after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time , incubation time and co - culture time , we set up the system of high frequency transformation of tomato cotyledons . then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation , and the resistant plants to hyg were obtained . by pcr analysis on part of the putative transformants , we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants . 5 . transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg . trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection , growth and bio - morphology analysis , hyg - resistant experiment and pcr analysis
  通过pcr检测证实部分番茄抗性植株中已导入hbmp - 3m基因；人骨形成蛋白一3成熟肤基因和全长基因分别转化番茄和烟草的研究5 .通过农杆菌介导法将hbmp一3全长基因导入烟草，并且获得了hyg抗性植株，通过gus基因瞬时表达检测、转化植株的生长情况及形态学分析、 hyg抗性鉴定及pc尺检测，证明目的基因己经整合到烟草基因组中。
• The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn
  用携带有几丁质酶基因和- 1 、 3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16 . 7 ，基因枪法的转化率为50 ，因此可能基因枪转化法更适于龙牙百合的遗传转化。