pcr reaction造句

• But the solutions used to do this can inhibit the PCR reaction.
• After PCR reaction, templates are denatured and disassociate from the beads.
• MAK2 assumes constant amplification efficiency during the PCR reaction.
• DNA polymerase in PCR reactions also results in blunt-ended PCR products.
• These structures significantly affect the primer efficiency and therefore the success of a PCR reaction.
• Very rarely does DNA contamination really affect the PCR reaction, at least in my experience.
• Gagneux performed about three PCR reactions for each stage of his analysis, while Constable might do double or triple that amount.
• Paired DNA and RNA molecules are comparatively stable at room temperature but the two nucleotide strands will separate above a primers for PCR reactions.
• However, use of the thermostable polymerase eliminates the need for having to add new enzyme to the PCR reaction during the thermocycling process.
• Just in case i had somehow messed up the reaction in the tube by adding the wrong thing, i ran my PCR reactions on a gel.
• Tetra-primer amplification refractory mutation system PCR, or ARMS-PCR, employs two pairs of primers to amplify two alleles in one PCR reaction.
• The only DNA detected in his specimens was identified as coming from the bacteria " Phyllobacterium mysinacearum ", which is a common contaminant in PCR reactions.
• DMSO in a PCR reaction is applicable for supercoiled plasmids ( to relax before amplification ) or DNA templates with high GC-content ( to decrease thermostability ).
• A triplex tetra-primer ARMS-PCR method was developed for the simultaneous detection of C677T and A1298C polymorphisms with the A66G MTRR polymorphism in a single PCR reaction.
• In a multiplex PCR reaction, it is possible for the different sequences to suffer from different inhibition effects to different extents, leading to disparity in their relative amplifications.
• After this initial construction phase, additional primers encompassing both ends are added to perform a regular PCR reaction, amplifying the target sequence away from all the shorter incomplete fragments.
• Mitochondrial DNA analysis might be susceptible to contamination by nuclear mitochondrial DNA sequences co-amplified during the PCR reaction, thus confounding the analysis of homoplasmic versus heteroplasmic mitochondrial DNA.
• A final concern is that bisulfite treatment greatly reduces the level of complexity in the sample, which can be problematic if multiple PCR reactions are to be performed ( 2006 ).
• However, whenever I do any type of PCR reaction ( whether it's mutagenesis or straight up generating amplicons for subcloning ), I always make the master mix " without " the enzyme.
• An extension of this approach includes an accurate model of the entire PCR reaction profile, which allows for the use of high signal-to-noise data and the ability to validate data quality prior to analysis.