pre-incubated造句

• In order to investigate the role of mannose receptor ( mr ) of human sperm , the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography . the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope , respectively . the mice were immunized with pmr and the antiserum was raised . after capacitation and induction of the acrosome reaction , the human spermatozoa and oocytes were incubated with the antiserum . then the sperm penetration assay was undertaken
  为了进一步探讨人精于mr在精卵融合中的作用，本文采用改良后的甘露糖-琼脂糖凝胶亲和层析法分离纯化人精子mr ，并将提纯的人精子甘露糖受体( purifiedmannosereceptor , pmr )作用于去透明带的金黄地鼠卵母细胞，运用透射电子显微镜技术和罗丹明偶联的兵豆凝集素( tritc - lca )免疫荧光标记技术观察pmr对卵子的影响。
• Based on epidermal strip bioassay , microinjection , patch - clamp and laser scanning confocal microscopy in the experiments , we provided the first evidence that map kinases , including mek1 / 2 or p38 / hog1 , plays an important role i n aba - or sa - induced h2o2 signal initial , amplification and specific targeting in response to stimuli in guard cells . aba - or h2o2 - induced vicia faba stomatal closure . was inhibited or reversed by the specific inhibitor pd98059 of mek1 / 2 ; the guard cells were pre - incubated or - microinjected by 10 umol l - 1 pd98059 , aba could not enhance the fluorescence intensity of h2o2 probe dichlorofluorescein ( dcf )
  在对照实验中， aba诱导荧光迅速增高；单独的pd98059 、 pd98059和aba共同处理气孔时，保卫细胞内h _ 2o _ 2探针荧光强度没有增高；将pd98059注射进入其中的一个保卫细胞，再以aba处理，使得两个有同样荧光基础的保卫细胞荧光强度对比强烈；将pd98059显微注射进入已被aba诱导dcf ( dichlorofluorescin )荧光强度升高的气孔保卫细胞，荧光强度下降，而没有被注射一边的保卫细胞中的dcf荧光强度不变。
• Winners will be entitled to present the awarded business plan to world - class venture capitalists . what s more , attendees may also be eligible to join the cyberport digital entertainment incubation - cum - training centre cyberport incutrain centre as the potential incubatees or pre - incubatees . details will be subsequently released in cyberport official website
  是次比赛将于9月1日至10月31日期间举行，优胜者可向世界级投资者推销其胜出的创业计划；更有机会获选为新成立之“数码港数码娱乐培育暨培训中心* ”的培育企业之一。
• Winners will be entitled to present the awarded business plan to world - class venture capitalists . what s more , attendees may also be eligible to join the cyberport digital entertainment incubation - cum - training centre ( cyberport incutrain centre ) as the potential incubatees or pre - incubatees . details will be subsequently released in cyberport official website
  是次比赛将于9月1日至10月31日期间举行，优胜者可向世界级投资者推销其胜出的创业计划；更有机会获选为新成立之“数码港数码娱乐培育暨培训中心”的培育企业之一。