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成熟蛋白的英文

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"成熟蛋白"怎么读用"成熟蛋白"造句

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  • maturation protein

例句与用法

  • Expression of gene coding mature peptide of sag1 of toxoplasma gondii in yeast pichia methanolica
    1成熟蛋白编码区基因在甲醇酵母中的初步表达
  • The epsp synthase is a necessary enzyme of the shikimate pathway and it is very conservative
    Epsp合成酶是莽草酸途径的一个关键酶,其成熟蛋白在进化中极为保守。
  • Comparison of the entire derived peptide sequence with other serine protease revealed significant homology , especially with a reported gene from another b . pumilus strain tyo - 67 ( 99 % homology )
    将克隆到的碱性蛋白酶基因(命名为ap )编码的成熟蛋白n一端序列与纯化的脱毛蛋白酶n一端序列进行比较,二者完全相同。
  • This pcr product was cloned into a t - vector and resulted in a plasmid ply11 , which was used as the template to generate a rna probe of halocin c8 for screening the halc8 gene in the as7092 genome by southern blot analysis
    将这段pcr产物克隆到t -载体上,形成质粒plyn ,测序结果表明pcr产物所编码的氨基酸是与halcs成熟蛋白的n端氨基酸序列是一致的。
  • It is deduced to encode 446 amino acids with the transpeptide of 98 amino acids . the amino acid sequences of their mature proteins are completely homologous . they also share 87 . 4 % identity with wheat
    推导两者均编码446个氨基酸,其中转运肽序列长98个氨基酸,两者成熟蛋白的氨基酸序列完全相同,与已知vde成熟蛋白的同源性在75以上,其中与小麦的同源性最高,达87 . 4 。
  • The cdna contains 947 nucleotides that codes for a precursor protein of 216 amino acids . sequence analysis shows that the n - terminal 1 - 25 amino acid sequence is a predicted signal peptide and other 26 - 216 amino acid sequence is a mature peptide which has no transmembrane domain
    Ecbp21cdna全长947bp ,编码216个氨基酸,序列分析显示其n端1 - 25氨基酸为信号肽, 26 - 216氨基酸为成熟蛋白肽段,并且不具有跨膜结构域。
  • The - glc squence of tea plant consists of 1475 bp , including 189bp 3 ' utr , 233bp 5 ' utr and 1050bp open reading frame . the deduced amino acids ( aa ) sequence is a 350aa - glc precursor which consists of an 82aa transit peptide and a 257aa mature protein
    结果表明,该序列的3 ’ -端和5 ’端的非编码序列长度分别为189bp和233bp ,开放阅读框为1050bp ,编码350氨基酸,其转运肽长度为82个氨基酸,成熟蛋白由257个氨基酸组成。
  • In this study , we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene . about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector . recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing . next , we construct recombinant plasmid pproex ? t - il - 2 . the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector . the recombinant plasmid pproex ? t - il - 2 was transformed into e . coli dh5a and the bacteria was induced with iptg . it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e . coli dh5a . the expression level was up to 30 % of the total bacterial proteins . the purified protein was used to prepare the antibody against chicken il - 2 protein
    经酶切鉴定及dna序列测定,该基因为鸡il - 2基因,其序列与sundick等报道的完全一致。在此基础上,我们把鸡il - 2基因亚克隆到大肠杆菌原核表达载体pproex ~ ( tm ) ht中,构建重组表达质粒并进行确证性序列测定,重组质粒测序结果表明将编码鸡il - 2成熟蛋白的基因正确地插入到原核表达载体pproex ~ ( tm ) ht的目的位点。重组质粒转化受体菌dh5后用iptg于37进行诱导培养, sds - page和westernblot分析显示,表达的鸡il - 2融合蛋白分子量约为18kda ,表达的融合蛋白经薄层扫描发现目的蛋白表达量约占菌体蛋白的30 。
  • According to the published data , three primers had been desighed and synthesized , xyndj - s including siginal sequence and xyna ! - w non - including siginal sequence had been cloned by pcr . according to the determined n - terminal amino acids sequence of xynb and the c - terminal conservative amino acids sequence of g / ll family xylanase
    Xynbn端十个氨基酸序列与已发表的最高同源性为90 ,根据n端序列测定结果及c端保守序列分别设计了两段引物,经pcr扩增出编码xynb成熟蛋白的核苷酸序列。
  • The p33 , which is considered to be the precursor of p27 and coordinate to e . coli tgi expressed phl , is expressed and excreted at lag phase . then a splicing process is supposed occurred during the exponential phase resulting in production of mature phospholipase p27 . the p32 p31 p30 , p29 are believed to be intermediates of this splicing process
    但在菌体生长进入稳定期后,仅产生p60和p27两种成熟的磷酯酶,其中p33为p27的前体蛋白,与phl应为同种蛋白,经剪切作用产生一系列的中间产物p32 、 p31 、 p30 、 p29 ,最终产生成熟蛋白p27 。
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