The entire halcs gene is located on a megaplasmid and contains a 849 - bp open reading frame that encodes a polypeptide of 283 amino acids . the promoter is typically haloarchaeal , but the start site of transcription is only seven bases from the 5 ' end of the initiator aug codon , making the halcs transcript another example of a " leadless transcript " in the haloarchaea 它的一29一24位置上是一“毛幻人” box序列,为典型的嗜盐古菌启动子;它的转录起始位点距离“ atg ”只有7个核普酸,也是典型的嗜盐古菌的“卜adless腼script ’ ’ 。
It is suggested that 538bp sequence of ast gene 5 " end had been cloned after the 138bp fragment was linked up with the 706bp fragment . the analysis of 538bp sequence with the software of promoter prediction indicated that there maybe exist four transcriptional initiator sites , one caat - box and two gc - boxes 将该片段与706bp的片段对接后,表明克隆到了ast基因的上游启动子的538bp序列,通过promoterpredict软件进行启动子的分析,显示该序列存在可能的四个转录起始位点,一个caat框和两个gc框。
In the part i , the sequence of the cloned promoter osg6b " was first analyzed . osg6b " had a whole length of 1688 bp and 98 % homology to known sequence of promoter osg6b . its transcriptional start point , tata boxes and the consensus sequences " tgtgg " conserved usually in anther - specific promoters were identical to those of reported osg6b 第一部分:对已克隆的启动子osg6b ’进行的序列分析表明, osg6b ’全长1688bp ,与报道的osg6b有98的同源性,两者在转录起始位点、 tatabox及其它花药特异性启动子共有的保守序列tgtgg完全相同。