In order to construct plant artificial chromosome , plasmid vectors have been constructed to integrate necessary elements into both right and left yac arms 为了构建植物人工染色体,我们构建了可以和含有着丝粒序列的yac载体的左臂和右臂进行同源重组的质粒载体。
Using a pair of special primers , whole sequence of m gene of b95 were amplified by rt - pcr and be sequenced after being directly inserted in plasmid vector pbluescript sk 结果表明: b95m蛋白基因全长1232bp ,共编码364个氨基酸,其中碱性氨基酸有47个,酸性氨基酸有30个,等电点为9
Furthermore , we discussed the influence of immune - sensibilization before induction to the activity of t - cell vaccine , compared the immune - sensibilization difference between hcv - adenovirus vectors and plasmid vectors 此外,我们还探讨了诱导前免疫致敏对t细胞疫苗活性的影响,并比较了hcv腺病毒载体和质粒载体在此免疫致敏功能方面的差异。
It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0 . 5 - 3kb fragments of a . brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors Brasilensesp7的nifa全序列构建在pgbd - c2载体上,得到重组质粒pgbd - nifa ,以其为诱饵,筛选sp7质粒基因组文库(构建在pgad系列质粒上) 。
Two primers were desighed and synthesized . xynbj coding nature protein of xynb have been cloned by pcr . then xynd1 - s nyna ] - w and xynb1 were all cloned into the plasmid vector pet - 22b ( + ) , and were all expressed in e coli 将此序列克隆进表达载体pet - 22b ( + )上,在大肠杆菌bl21 ( de3 )中也得到特异性表达,并有正常的生物学活性,证明了基因xynb1的生物学功能。