According to the research of physiological and biochemical indicators or index , components of soluble proteins , substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l . , we tried to find the law of the physiological and biochemical response of brassica oleracea l . leaf to low temperature . at the same time , discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress . the results are showed as follows : malondialdehyde ( mda ) , superoxide dismutase ( sod ) , ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature 本文以甘蓝叶片为材料,通过对低温5胁迫下甘蓝生理生化指标、可溶性蛋白组分以及磷酸化底物蛋白、蛋白激酶活性的研究,以期找出甘蓝叶片对低温胁迫的生理生化响应规律,为甘蓝露地越冬栽培防范寒害提供理论指导,同时对低温胁迫下甘蓝逆境信号传导进行了探讨,从而为彻底弄清低温胁迫机理提供进一步的证据,研究的主要结果如下:丙二醛含量( mda ) 、超氧化物歧化酶( sod ) 、抗坏血酸过氧化物酶( asp )和过氧化物酶( pod )活性在低温处理0 30min发生显著变化,低温处理3min后,甘蓝叶片内mda含量基本没有变化,处理5min时出现第一个峰值,达到对照的104 . 10 , 10min出现低谷,仅为对照的86 . 27 ,随后再次上升, 30min时超过第一峰值,为对照的113 . 93 。
The method of ctab was specifically improved for the extraction of genomic dna and total rnas from brassica oleracea l . and brassica napus l . 2 . arclboler gene was amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 以甘蓝( 200110197 )和油菜( y578 - 2 )自交不亲和系的植株为材料,提取幼苗中的gdna及花蕾期的柱头、子房、花瓣、叶片、根的mrna ,并对mrna进行反转录合成cdna ,然后分别以gdna与cdna为模板扩增arc1蛋白编码序列。
However , there are still some problems that cannot be avoided , such as construction of full - time supervising system for qualitative safety are lagged ; it lack the service center for science , technology and innovation , so it is too slow to generalize the use of a standard technology ; the contradiction of planting structure are relatively obvious , the comprehensive motivation of industry are too weak ; the construction of information service system are not so perfect ; etc . in order to solve the problem that limited the progress of industrial development of brassica oleracea l . var 但也存在一些无法回避的问题,如全程质量安全监控体系建设滞后;缺少科技创新服务中心,标准化技术推广应用缓慢;种植结构性矛盾比较突出,产业综合带动力弱;信息服务体系建设不够完善,等等。论文针对台州西兰花产业发展过程中存在的问题,进一步分析了促进西兰花产业发展的具体对策和措施: ( 1 )利用wto规则,依靠科技创新,全面提高西兰花出口的综合竞争力。
In thl1 gene , there are some famililar enzyme digest sites including bamh i , hind , sac i and sal i . the sequence of thl1l from brassica oleracea l . shows 99 % identity to the sequence of thll from brassica napus l . there are some phosphorylation sites and an active site of thoiredoxin h members consisting of cppc in thll protein 甘蓝和油菜thl1基因序列有5个碱基的差异,而氨基酸仅有2个氨基酸的变异,同源性达99 。在thl1蛋白的氨基酸序列中发现了一个硫氧还蛋白家族的活性位点( cppc )和多个磷酸化位点。
Brassica oleracea is the species of plant that includes many common foods as cultivars, including cabbage, broccoli, cauliflower, kale, Brussels sprouts, savoy, and Chinese kale. In its uncultivated form it is known as wild cabbage.