perfusing造句

material and methods normal rats of male sd were divided into young, adult, and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0.5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50-100 ml and then 4 % paraformaldehyde pbs 100-400ml were perfused through the left ventricle of the heart, the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration, dipping into paraffin for embeding, and reshaping the pieces
2）磷酸缓冲液100400m，30分钟灌注完毕，取出整脑，在上述固定剂oc）内后固定12小时。切取观察部位脑块，然后，进行梯度酒精脱水，浸蜡，包埋，修块，石蜡连续切片（德国leica石蜡切片机人切片厚度still，zlllll，蛋自甘油载片捞片，60c烤箱过夜，二甲苯脱蜡，梯度酒精置换，浸水，h六染色，梯度酒精脱水，二甲苯透明，中性树脂封片。室温风干后，显微镜观片，olympus万能显微镜照相。
It's difficult to find perfusing in a sentence. 用perfusing造句挺难的

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