rnase中文是什么意思

• 核糖核酸酶

• rnase a    rna酶a
• rnase t    rna酶t
• pancreatic rnase    胰rna酶
• rnase d    rna酶d
• rnase h    rna酶h; 核糖核酸酶h
• rnase i    rna酶Ⅰ
• rnase inhibitor    rna酶抑制剂
• rnase p    rna酶p; 核糖核酸酶p
• rnase protection    rna酶保护术
• rnase ribonuclease    核糖核酸酶
• türnase    车门锁钩
• placental rnase protein inhibitor    胎盘rna酶蛋白抑制剂
• rnase n1 and n2    rna酶n1和n2
• rnase protection analysis    核酸酶保护分析
• rnase protection assay    核糖核酸酶保护测定
• rnarna三联体密码    rna coding triplet
• rnanucleotidyl transferase    核糖核酸核苷酸转移酶
• rnallhart    黑道新鲜人
• rnakai    吉林鹅观草
• rnaheterogeneous nuclear rna hnrna    核内不均一

• The first part of our experiment is to extract and purify the total rna from the human liver tissue in the condition without rnase and to identify its integrity and purity
  本研究第一部分包括肝组织中总rna的分离提取； rt - pcr扩增出血管抑素基因。首先，采集术后新鲜肝组织，并于无rnase的条件下分离纯化总rna 。
• Angiogenin belongs to a protein superfamily of rnase . it is the only member being able to stimulate angiogenesis . on the other hand , angiogenin is also the only angiogenic factor known to exhibit ribonucleolytic activity
  血管生成素是核糖核酸酶超家族中唯一一个具有促血管生成能力的成员，也是目前已知的所有血管生成因子中独具核糖核酸酶活性的因子。
• This modification includes : ( 1 ) selecting two important molecules as candidates , ( 2 ) choosing a promiscuous t - cell epitope , and two b - cell epitopes or conserved amino acid sequences from the two important molecules , ( 3 ) connecting them adequately through analysis by the molecule designing software . therefore , the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing . based on the molecule designing methods , the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse , as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
  本研究以蛋白质分子设计的理论和方法研究避孕疫苗，将sp17和cyritestin关键表位和牛核糖核酸酶非选择性th细胞表位合理组合，获得新抗原- 35肽序列；并在合成、纯化后分别与弗氏佐剂、免疫刺激复合物( iscoms )混合后免疫不同遗传背景的雌性小鼠，观察血清和生殖道内的特异性抗体滴度的动态变化、生育力的改变以及免疫后小鼠重要脏器的组织病理学改变：以及在ivf下，新抗原的特异性抗血清对精卵相互作用的影响及抗原在精子表面的特异性定位。
• Firstly , the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector . comparing the eo sequences with other strains eo by biosoftwares , dnastar5 . 0 and bioedit , the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii . two amino acid streches of 15 csfv strains eo , which form the rnase active site , and histidine residues essential for rnase catalysis in both ones were highly conserved . the eo protein propertys of antigen epitope , hydrophobicity , isoelectric point were also predicted by bioinformatic method
  首先，采用rt - pcr和nest - pcr技术扩增了csfv - jl株和csfv - ln9912株eo基因，插入teasy载体后测序，应用dnastar5 . 0和bioedit软件将其与本实验室已测其它毒株和genebank中登录的csfv代表毒株eo基因序列进行比较，绘制了遗传进化树并预测了eo蛋白的抗原表位、疏水性、等电点等特性。
• The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method , then the total rna digested by dnase that had not rnase was used for rt - pcr . i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition . at the end i selected the magnesium ion density as 1 . 25 mm . the production of rt - pcr was inserted directionally into pgem ? z ( ampr ) . the pgem ? z ( ampr ) was used to transform e coli jm109 . i got a positive clone through culling and identificatin . the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
  分取诱导培养液中的菌体，用异硫氰酸胍法提取总rna ，总rna再经无rna酶的dna酶处理后用于rt ? pcr 。在pcr扩增目的基因时，通过优选扩增体系，使镁离子浓度为1 . 25mm时rt ? pcr可顺利地获得目的基因，并能定向克隆到载体pgem ? 3z ( amp ~ r )中。用克隆载体转化宿主大肠杆菌jm109 ，通过筛选获取阳性克隆子，对阳性克隆子进行酶切与pcr鉴定，并对载体中插入的目的基因进行测序。
• Secondly , we established a rdrp reaction system . then the recombinant ns5 protein was added to the system and its rdrp activity was confirmed after the newly synthesized rna showed resistant to rnase a . furthermore , we found that the recombinant ns5 could specifically bind antibodies in anti - den serum indicating the immunoreactivity of the recombinant ns5 and the irnmunogenicity of den ns5
  重组nss蛋白的rd即反应和反应产物的rnasea敏感性实验表明，所得重组nss蛋白具有良好的rdrp活性，能以亚基因组rna为模板合成与之互补的rna ，新生链是与模板链相结合的双链形式存在于rd即反应体系中。

• rnaseとは意味：

  RNase {略} : ribonuclease RNA分解酵素、リボヌクレアーゼ