After careful studying their relative importance to immune response and the possibility of the match , seventeen sequences of interest were selected for further experiment , including estss analogous to 11 . 5kd antibacterial peptide , lysozyme , serine protease and its inhibitor , lectin , antifreeze protein , et al . primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library 在仔细分析了它们在免疫系统中的重要性和在对虾中出现的可能性之后,从中选出了17条可能编码抗菌肽,溶菌酶,凝集素、丝氨酸蛋白酶及其抑制剂,抗冻蛋白等蛋白质的序列,以此为依据设计引物,在中国对虾的血液和头胸部cdna文库中扩增相应的序列。
A lectin named pclii with multi - functions was isolated from the rhizomes of polygonatum cyrtonema hua , a liliaceae plant used as traditional chinese herbal medicine . pclii can bind mannose and silia acid , it also can agglutinate rabbit erythrocytes and gastric tumor cell lines sgc and hsc strongly and inhibite the growth of some pathogens . pclii is a strong mitogen toward human t - lymphocytes and an efficient ca2 * antagonist ( ca ) or ca2 + channel blocker ( ccb ) 百合科植物囊丝黄精( polygonatumcyrtonemahua . )是我国传统中草药,其根状茎中含有一种凝集素,命名为黄精凝集素( polygonatumcyrtonemahua . lectin ,简称pcl ) ,具有特异性结合甘露糖和唾液酸以及凝集兔红细胞、促t细胞有丝分裂等活性。
Numerous mushrooms are toxic to insects . to identify the chemicals involved in insecticidal activity , the toxicity of 14 species has b een studied for water solubility , thermolability , and dialysis . the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects . among proteins , lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease 许多蘑菇都对昆虫表现出毒性.为了证实与杀虫毒性有关的化合物,对14种蘑菇的毒性在水溶性、热敏性和可透析性等方面进行了研究.研究数据表明,蛋白质对大多数蘑菇子实体的杀虫活性起着重要作用,也许是一种可以用于植物抵抗害虫的基因源.在数种蛋白质中,凝集素和溶血素因不受蛋白酶的影响而成为良好的杀虫剂候选材料
To date there is no specific database for toxin and anti - nutrient proteins . in order to establish such a database , we have collected data from some nucleotide and protein database available at present . totally , 1033 toxin proteins , including 172 from plants , 251 from animals , 577 from bacteria and 42 from " other organisms , as well as 1013 lectins and 391 proteinase inhibitors are collected 本文通过对主要基因或蛋白数据库进行检索,收集散落于不同基因或蛋白数据库中的毒蛋白氨基酸序列数据1033个,其中植物毒蛋白172个,动物毒蛋白251个,细菌毒蛋白557个,其它生物如真菌、藻类等的毒蛋白42个;抗营养因子蛋白数据1404个,其中凝集素1013个,蛋白酶抑制剂391个。
A page gel , stained by the petiodic acid - schiff ' s method to reveal glycoproteins , further displayed that the bindng - protein was a glycoprotein belonging to lectin , which contained 17 . 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method . peptide mapping was comparable to the reported protein in protein bank . the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase , aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b , glutamyl - trna reductase , histidyl - trna synthetase 连续梯度聚丙烯耽胺凝胶电泳、 sds一聚丙烯酞胺凝胶电泳和等电聚焦的结果表明该蛋白分子量为1 “ . skda ,由二个相同的亚基组成,亚基分子量为” . ikda ,等电点为8 . 25 .糖蛋白染色结合考马斯亮蓝染色的结果证实此结合蛋白是个糖蛋白,其中糖含量为17 . 44 % ,蛋白含量为82 . 56 % .凝集反应确定该糖蛋白也是一个凝集素
A web - based blast is also constructed to facilitate the process . and the contigs and ests sequences are formatted as subject database . by similarity search , about 442 estss were found possibly encoding immune related proteins , such as lysozyme , lectin , serine protease , serpin , thioredoxin , sod and so on 为了方便相似性比对,在由四台pc机并行组成的机群( pc - cluster )上将blast程序和interproscan程序本地化,并且将contig和ests序列文件做成blast程序中的序列库文件以供搜索。
The confromational changes of polygonatum cyrtonema hua . lectin ii modified by reagents were studied by fluorescence spectrum and the fluorescence quenching study on pclii with cscu ki and acrylamide showed they all quenched the fluorescence of lectin through dynamic quenching mechanism . the antiviral activity of polygonatum cyrtonema hua 当受试细胞系为ht - 4时,黄精凝集素n对王a从1和fa琴2的半数抑制浓度均为ec50 = = o . 08u岁nil ;当受试细胞系为cem ,黄精凝集素n对班v