demecolcine中文是什么意思

中文翻译手机版

After 15h of maturation . 98 % of oocytes released the first polar body and thus the first meiosis of mouse oocytes ended . secondly , strong chromosome to chromosome binding was induced by culturing pro - mi oocytes in demecolcine supplemented medium
第一次减数分裂期小鼠卵母细胞经化学去核后，去除透明带，并胎儿成纤维细胞粘合、电融合并srcl2激活，培养于微孔中。
Since the success of dolly , the first cloned sheep with the adult somatic cells as karyoplast donor , new approaches have been developed for nuclear transfer technology . here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture . enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
将培养10h的化学去核卵母细胞与供体成纤维细胞融合后lh 、 2h 、 3h ，分别有77 . 6 % 、 70 . 6 % 、 58 . 9 %重构胚的染色质发生凝集，其余胚胎的染色体则处于原核期;而只在融合后3h ， 27 . 9 %重构胚被标记出组装的纺锤体，且其中的同源染色体己经分离。