hybridoma造句

• Anti - melatonin monoclonal antibodies of higher titer , affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections . we obtained 6 strains of hybridoma , all of them secreting specific antibodies to mt , we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique . an inhibition curve for mt was obtained in the range of 50pg to30ng , and 1 . 4ng of mt inhibited the value of the assay by half . we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues , the moabs recognized mt but
  通过将mt用甲醛作连结剂连结到牛血清白蛋白上sa采用皮下多点注射兔疫小鼠得到了高效价，高亲和力，较好特异性的抗mt单克隆抗体，最后获得了5株单克隆细胞株，都能分泌针对mt的特异性抗体，建立了选择性基团免疫分析法，用制备的抗体测定了人血清中mt的含量，作了mt的抑制标准曲线，其抑制范围从50pg ? 30ng ，半抑制量为1
• 2 - e4 - a and 82 - 6 are hybridized during their log growing time , and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells . the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted , and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared . the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody , indirect elisa for anti - p24 antibody , and rbc solid - phase adherence for bispecific antibody
  选其中一株3 - h _ ( 11 )做杂交-杂交瘤细胞染色体计数，同时计数两母株2 - e _ 4 - a和s _ 2 - b的染色体数：制备腹水型和上清型抗体，用三种方法同时检测其中的双特异性抗体、单特异性抗人红细胞抗体和抗p24抗体，即红细胞固相吸附法测双特异中文摘要性抗体，红细胞凝集试验测单特异性抗人a型红细胞抗体，间接elisa法测抗p24抗体；用腹水型抗体做耐热性及耐冻融实验。
• Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
  Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。