hybridoma造句

• By this procedure, milstein and kohler developed a hybridoma that made a specific antibody .
  米尔斯坦和科勒用此方法培养出一个杂种瘤，后来成为一种特效抗体。
• The batch culture of jal1 hybridoma cells
  1杂交瘤细胞的批量培养研究
• 1975 hybridoma technology deeloped for production of monoclonal antibodies
  1975年,杂交瘤技术被应用于生产单克隆抗体
• 1975 hybridoma technology developed for production of monoclonal antibodies
  1975年,杂交瘤技术被应用于生产单克隆抗体
• Development of hybridoma cell line excreting monoclonal antibody against total aflatoxins
  抗总黄曲霉毒素单克隆抗体的制备及特性
• 2 . monoclonal antibody gl biochem provides the following hybridoma development program
  吉尔生化从2005年2月起开始提供抗体制备服务。
• The isolated at - iii will be used in the screening of positive clones of hybridoma cell lines
  本研究采用常规法进行小鼠免疫和细胞融合。
• Establishment and characterization of two hybridoma cell lines secreting anti - osteosarcoma antibody
  抗人成骨肉瘤杂交瘤细胞系的建立及其特性鉴定
• The chromatosome of hybrid - hybridoma 3 - hn are 132 + 15 , that of hybridoma 2 - e4 and s2 are 84 + 9 and 89 + 9
  2 e4和s 。在hat液中培养，均于一周内死亡。
• Expression of human papillomavirus type 16 l1 and construction of hybridoma cell strain of human papillomavirus type 16 l1 monoclonal antibody
  1蛋白表达及其单克隆抗体杂交瘤细胞株的建立
• The ascites fliud titer of the other hybridoma s2 for further hybridization is 1 : 16000 by blood agglutination . the isotype of 2 - e4 is igg2a , and s2 is iggs
  用于再杂交的另一细胞株为本室保存的分泌抗人a型红细胞单克隆抗体的杂交瘤细胞株s ； ，其血凝效价为l ： 16000 。
• Methods 1 mr1 hybridoma was cultured in hollow fibre system . culture supernatant was harvested , then precipitated by half - saturated ammonia sulphate . mfm was purified by deae - sephacel ion exchange chromatography . mri concentration was measured by elisa
  硫酸钱半饱和沉淀， oeae阴离子交换层析分离纯化抗co40l单克隆抗体mri 。 pbs透析后， 505一队ge检测纯化抗体的纯度， eusa测定抗体浓度。
• Therefore the hybrid - hybridoma can secrete not only bispecific antibody but also anti - human rbc type a monospecific antibody and probably anti - p24 monospecific antibody . the mixed antibodies purified by ammonium sulfate is further purified by p24 affinity chromatography . the unsp
  这样，分离出双特异性抗体，并证实杂交杂交瘤除分泌抗p24和人红细胞的双特异性抗体外，还分泌抗p24单特异性抗体和抗人a型红细胞单特异性抗体。
• Methods : in the first part , we survived and cultured m4g3 hybridoma in vitro . after two weeks of injecting hybridoma into mouse abdominal cavity , we collected abundant ascites . at the same time , 4 strains of breast cancer cells had been growing in vitro , digested and collected for immunocytochemistry and western blotting with m4g3 ascites
  方法：第一部分：复苏m4g3杂交瘤细胞，体外培养，小鼠腹腔注射，进行单克隆抗体的大量制备；复苏t47d等四种乳腺癌细胞，体外培养，爬片，应用m4g3进行免疫细胞化学实验；四种细胞收集，应用m4g3进行western - blot染色。
• Serious cross reation existed between v . albo - atrum and mv2 , mv3 , mv4 . the other pathogen isolates v31 and v32 also had cross reactions , but the reaction was not serious . because limited number of pathogen isolates were selected , it could not prove that the selected immunogen was widely presentative , more pathogens isolates should be tested to verify the acquired hybridomas cells
  5株单抗杂交瘤细胞中没有一株具有种或属的特异性，其中mv2在棉花黄萎病菌若干菌系间的检测表明其能够区分不同的致病类型； mv1和mv4组合检测的结果基本上能将棉花大丽轮枝菌鉴定到种；黑白轮枝菌与mv2 ， mv3 ， mv4的交叉反应比较强烈，其他菌株v3 ， v32有个别的交叉反应，但不强烈
• Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
  利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠，然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ，进行其分泌抗meq单克隆抗体( mcab )能力的筛选。
• Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe . wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ) , and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution
  该纯化cpe对小白鼠的半数致死量为2 . 5ug /只；该cpe豚鼠皮肤蓝斑单位为2500 4000 ；引起新西兰白兔小肠积液的最小毒素量为25ug 。将纯化的cpe ，在含0 . 4福尔马林的的pb液中透析制备类毒素。
• The bispecific antibody tilers of ascites fluid and culture supernatant of 3 - hn are 1 : 16000 and 1 : 64 . at the same time the anti - p24 antibody and the anti - human rbc type a antibody in the ascites fluid of hybrid - hybridoma 3 - hn are tested , and their titer is 1 : 800 and 1 : 16000 respectively
  为89士9条j十； ；的腹水型和上清型双特异性抗体的效价分别为1 ： 16000和1 ： 64 ，同时测定腹水型抗体抗p24抗体活性及抗人a型红细胞凝集抗体活性，效价分别为1 ： 800和1 ： 16000 。
• Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months . the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg , and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa . through three clonings less diversed anti - p24 hybridoma cells are gained
  方法：基因工程p24抗原免疫小鼠4次，历时2个月，取脾细胞与骨髓瘤细胞株sp2 0 ，用peg融合， hat选择培养和间接elisa筛选分泌抗p24抗体阳性的杂交瘤细胞，三次克隆化后得稳定分泌抗p24抗体的杂交瘤细胞株。
• In the present study , the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis . total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies . after obtained using rpas system , vh and vl genes were used to assemble scfv gene fragment with a linker primer
  应用重组噬菌体抗体库技术，从分泌小鼠抗牛精子sp18抗体的杂交瘤细胞系中分离总rna ，克隆抗体重链和轻链可变区基因，加入连接肽引物( linkerprimer )组装成单链抗体scfv ( singlechainfragmentvariable )基因并用rs引物进行扩增， sfi 、 not酶切，回收后与pcantab5e载体相连，转化e . colitg1宿主菌，构建单链抗体文库。