开放阅读框架的英文

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用"开放阅读框架"造句

open reading frame

open reading-frame

开放: come into bloom
阅读: read
框架: frame; framing; sh ...

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例句与用法

Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat . its gene is located on chromosome 2q21 containing 17 exons . gnt - v protein is encoded by exons 2 - 17 as open reading frame
人类gnt - v由741个氨基酸组成，有6个潜在的n -糖基化位点，基因定位于染色体2q21 ，含有17个外显子，其开放阅读框架由外显子2 - 17进行编码。
Phaa , phab and phac were amplifed from the subclone of pseudomanas sp . producing pha by pcr . the gel electrophoresis analysis showed that the molecular weights of cloned phaa , phab and phac were equal to fragment speculated from three orfs
利用所提取的开放阅读框架的序列设计三对引物，采用pcr技术，从合成pha的亚克隆片段中分离出phaa 、 phab和phac三个基因片段，经凝胶电泳分析表明，所克隆的三个基因分子量大小与推测的三个开放阅读框架中基因片段大小一致。
The 3 . 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively . through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported , we found that there are four intact open reading frames in the 3 . 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported , and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames . this open reading frame located on about 5 . 5kb upstream of the nfec gene , which includes 207bp nucleotides , coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported
本研究首先利用转座子tn5gusa5对重组质粒pgxn201进行诱变，获得3 . 4kbecori片段插入了tn5gusa5的突变质粒pgxn215 、 pgxn216 、 pgxn217 。对这些突变质粒进行亚克隆及测序分析并与基因库中已报道的慢生型大豆根瘤菌的dna序列作比较，发现突变质粒pgxn217中tn5gusa5恰好插入在一个未知功能的开放阅读框架内。将pgxn201的3 . 4kbecori片段与m13作连接，获得亚克隆pgxn201c ，对pgxn201c进行测序分析，发现与基因库中已报道的慢生型大豆根瘤菌的dna序列有95的同源性。
The cdna is 2 149 bp long with an open reading frame of 1 697 bp , which encodes a polypeptide of 565 amino acids , preprotein of 62 . 3 kda ; 5 " untranslated regions contains 59nt , 3 " untranslated regions contains 391nt , a poly ( a ) tail signal and long poly ( a ) tail
该cdna全长2149bp ，包含一个1697bp的开放阅读框架，编码565个氨基酸。在起始密码子上游有一个由59个碱基组成的5 ’非编码区；在终止密码子下游有一个由391个碱基组成的3 ’非编码区，包括4个分解信号、 1个加尾信号和1个长度为17个腺苷酸的poly ( a )尾。
In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l . , pichia methanolica high - level expression systems of the genes have been constructed , and the milligram expressed protein was purified using probond resin purification system , which may result in further identification of the function of the aba binding protein . the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr , 369 bp long 3 ' - utr and poly ( a ) tail . the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr , 144 bp long 3 " - utr and poly ( a ) tail
3 - 5 - race扩增片段序列分析结果表明， abp370扩增片段的全长cdna为3449核苷酸，其中5非翻译区为876个核苷酸， 3非翻译区为369个核苷酸并末端带poly ( a )尾巴，从起始密码子atg至终止密码子tga ，含有一个编码768个氨基酸残基的开放阅读框架( 2304bp ) ； abp640扩增片段的全长cdna为1012核苷酸，其中5非翻译区为88个核苷酸， 3非翻译区为144个核苷酸并末端带poly ( a )尾巴，从起始密码子atg至终止密码子taa ，含有一个编码260个氨基酸残基的开放阅读框架( 780bp ) 。