transformant中文什么意思

转化体

转化细胞

• transformant doctrine:    变化学说

例句与用法

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1. Transformation was done by electroporation . human fl extracellular domain cdna transformed to yeast host strain km71 , then his + muts phenotype transformant was screened out and cultured in flasks , and rhfl was expressed under the induction of 0 . 5 % methanol
   我们提取了km71ppic9k - fl转化菌株的基因组dna进行southern实验，检测目的基因的整和插入；提取总rna ，进行了northern实验，检测fl基因在转化菌株中的表达。
2. Transformant ybt - 833 - 1 kept all indigenous plasmids , ybt - 833 - 2 lost a plasmid carrying crylab and ybt - 833 - 3 lost all indigenous plasmids carrying icp genes . comparing their potency with natural strain ybt - 833 , the overall toxicity of all transformants was not noticeably higher than strain ybt - 833
   质粒pbmblc电脉冲转入菌株ybt - 833 ，得到了几个icp基因背景不同的转化子，它们的综合毒力较出发菌ybt - 833都没能得到提高。
3. The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
   将带有hwtx -基因的ppic9k经blgii线性化后，转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌，进一步用遗传毒素g418筛选多拷贝的转化菌株，命名为gh1 ；将gh1甲醇酵母菌用0 . 5的甲醇诱导表达，发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超滤膜超滤，再经cm阳离子交换， c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分，其中4289 . 05的组分经质谱鉴定，氨基酸组成分析和序列测定为正确的表达产物，生物学活性表明其活性为天然毒素活性70 % ，表达量为80mg / l 。
4. Plant expression vector lba4404 ( pbin + - 35s - cbf3 + pal4404 ) was used in the genetic transformation of cucumber and micro - tomato . establishment of high efficiency regeneration system and determination of parameters involved in agrobacterium - mediated transformation , screening and characterization of transformant were systemically researched
   将植物表达载体pbin ~ + - 35s - cbf3对黄瓜和小番茄进行遗传转化，建立了黄瓜高频再生和遗传转化体系，获得了一株转基因黄瓜植株，并通过了植物基因组pcr检测；获得了两个具有kan抗性的小番茄再生绿芽。
5. T . identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis . both analysis the target bands were observed . so the integration of the cpti gene into mustard genome dna was confirmed . the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants
   转基因芥菜植株的鉴定取再生苗的叶片提取dna ，进行pcr扩增和pcrsouthernblot分析，转化再生植株大部分呈阳性，而非转化的再生植株均为阴性，证明cpt基因已存在于芥菜基因组中。