pachia中文是什么意思

中文翻译手机版

The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3 , then the vector was transferred into pachia pastoris gs115 strain . the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation . sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
将缺失型pap基因克隆于酵母分泌型表达载体ppicgk构成重组载体，然后导入毕赤酵母（ p8chianastoris ）菌株gslls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行pap的表达，经sds page分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34ku ，经western blotting分析，该蛋白与法国pap抗血清有特异性反应，体外活性检测表明该蛋白对tmv的侵染性具有高度的抑制性，说明该pap基因在毕赤酵母gs中也得到了正确表达。