Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding-peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa . after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding . the amino acid sequence results shown three different sequences : c-rrpaqsg-c-nkhnrki-c and c-rgmsrki-c 在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆，elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列：c-rrpaqsg-c、c-nkhnrki-c和c-rgmsrki-c，其中优势克隆序列为c-rrpaqsg-c；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。
Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding-peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa . after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding . the amino acid sequence results shown three different sequences : c-rrpaqsg-c-nkhnrki-c and c-rgmsrki-c 在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆，elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列：c-rrpaqsg-c、c-nkhnrki-c和c-rgmsrki-c，其中优势克隆序列为c-rrpaqsg-c；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。
Assaying of the protective effect of mimotopes we divided the mimotopes into three groups based on their amino acid sequences " homology, with phage ml3 as the control group, then immunized 6-week balb / c mice, 10 mice per group . after amplifing positive phages and quantification, we used them to immunizing mice subcutaneously at 2-week intervals . the dose given is 100ug phage per mouse at first immunization and 30ug phage per mouse at the latter two immunizations 并且在这12个阳性克隆中，有8个可特异性的与hini多抗结合，可能为二者共有的抗原表位口2.模拟表位的免疫保护作用分析根据模拟表位的序列相似性分3组免疫6周龄的雌性balb/c小鼠，10只/组，以野生噬菌体为阴性对照。
Assaying of the protective effect of mimotopes we divided the mimotopes into three groups based on their amino acid sequences " homology, with phage ml3 as the control group, then immunized 6-week balb / c mice, 10 mice per group . after amplifing positive phages and quantification, we used them to immunizing mice subcutaneously at 2-week intervals . the dose given is 100ug phage per mouse at first immunization and 30ug phage per mouse at the latter two immunizations 并且在这12个阳性克隆中，有8个可特异性的与hini多抗结合，可能为二者共有的抗原表位口2.模拟表位的免疫保护作用分析根据模拟表位的序列相似性分3组免疫6周龄的雌性balb/c小鼠，10只/组，以野生噬菌体为阴性对照。
Recently, some new studies suggest that small cytokine peptide-mimics can function as the agonist or antagonist of cytokine receptor, and these small molecular peptides can be synthesized easily and oral adminstration with high concentration . based on these work, we have tried to set up experiment on tnfa-binding peptides and tnfa mimotopes which may be as leads of tnfa antagonist 近年来的研究发现，小分子细胞因子模拟物具有细胞因子受体激动剂或拮抗剂的作用，且小分子模拟物因容易合成并能够给予高浓度、可口服等优点使其具有更加广阔的研发与应用前景。
These phage display peptides may be the tnfa mimotopes . part iii specific c7c tnfa mimotope phage clone as target for screening of tnfa specific binding-peptides from 12mer phage display peptide library : by using the specific c7c tnfa mimotope as target, we developed a novel approach for screening phage peptide library targeting specific phage display peptides . we used c7c tnfa mimotope phage clone lcs-7 ( c-rrpaqsg-c ) as target to screen 12mer phage peptide library and identify the positive clones by phage elisa 3、以特异性噬菌体克隆为靶筛选肽库的研究??一种肽库筛选新方法的建立：为了深入研究细胞因于与配基（受体或抗体）相互作用的关键残基及其空间构象，在上述研究基础上，我们以tnfa表位模拟肽克隆为靶筛选噬菌体十二肽库，找寻与之结合的tnfa结合肽噬菌体克隆；并以此拓宽了噬菌体肽库的应用范围。
