mimotopes造句

• Screening of tnf-mimotopes from c7c phage display peptide library
  表位模拟肽的研究
• The result indicated that we had selected the mimotopes of influenza virus a . 2
  提示阳性噬菌体递呈肤可能为流感病毒的模拟表位。
• And the quantity of iggl was much more than the one of igg2a in mice serum, so we deduced initially that the main type of immune reaction that the mimotopes induced was cell-mediated immune response
  而血清中针对流感病毒的iggza含量明显高于iggi，初步推断模拟表位引起的免疫反应是以thl引起的细胞免疫为主。
• The result indicated that the mimotopes could induce part crossed protection against h1n1 . in conclusion, we acquired the cross-protective mimotopes of influenza virus a and explored a new way to reseach for the cross-protective epiptops
  通过以上实验，筛选到具交叉免疫保护作用的甲型流感病毒模拟表位，为流感疫苗的研制提供了新的思路和有益的线索。
• The result indicated that the mimotopes could induce part crossed protection against h1n1 . in conclusion, we acquired the cross-protective mimotopes of influenza virus a and explored a new way to reseach for the cross-protective epiptops
  通过以上实验，筛选到具交叉免疫保护作用的甲型流感病毒模拟表位，为流感疫苗的研制提供了新的思路和有益的线索。
• Part iv computer-aided molecule modelling of tnfa mimotopes and tnfa-binding peptides : to investigate the interaction between tnfa mimotopes and tnfa-binding peptides, the computational docking program autodock ( with confirming calculations using discover ) was used to predict the binding modes of llt-18 with tnfa firstly, then lcs-7 was docked to llt-18 by manual . the interaction between llt-18 and tnfa or lcs-7 showed electrostatic interaction and h-bond dominant
  进行对接，对两者间结合位点进行分析；在此基础之上，以insight11软件构建lcs刁分于三维结构，autodock程序对接llt和lcs7两个短肽分子，找寻两者结合的关键性残基，结果表明llt与tnfa及lcs7分子间相互作用以静电相互作用为主，lcs刀和tnfa活性部位的arg在分于间相互作用中起重要作用。
• Part iv computer-aided molecule modelling of tnfa mimotopes and tnfa-binding peptides : to investigate the interaction between tnfa mimotopes and tnfa-binding peptides, the computational docking program autodock ( with confirming calculations using discover ) was used to predict the binding modes of llt-18 with tnfa firstly, then lcs-7 was docked to llt-18 by manual . the interaction between llt-18 and tnfa or lcs-7 showed electrostatic interaction and h-bond dominant
  进行对接，对两者间结合位点进行分析；在此基础之上，以insight11软件构建lcs刁分于三维结构，autodock程序对接llt和lcs7两个短肽分子，找寻两者结合的关键性残基，结果表明llt与tnfa及lcs7分子间相互作用以静电相互作用为主，lcs刀和tnfa活性部位的arg在分于间相互作用中起重要作用。
• Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding-peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa . after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding . the amino acid sequence results shown three different sequences : c-rrpaqsg-c-nkhnrki-c and c-rgmsrki-c
  在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆，elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列：c-rrpaqsg-c、c-nkhnrki-c和c-rgmsrki-c，其中优势克隆序列为c-rrpaqsg-c；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。
• Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding-peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa . after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding . the amino acid sequence results shown three different sequences : c-rrpaqsg-c-nkhnrki-c and c-rgmsrki-c
  在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆，elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列：c-rrpaqsg-c、c-nkhnrki-c和c-rgmsrki-c，其中优势克隆序列为c-rrpaqsg-c；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。
• Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding-peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa . after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding . the amino acid sequence results shown three different sequences : c-rrpaqsg-c-nkhnrki-c and c-rgmsrki-c
  在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆，elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列：c-rrpaqsg-c、c-nkhnrki-c和c-rgmsrki-c，其中优势克隆序列为c-rrpaqsg-c；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。
• Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding-peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa . after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding . the amino acid sequence results shown three different sequences : c-rrpaqsg-c-nkhnrki-c and c-rgmsrki-c
  在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆，elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列：c-rrpaqsg-c、c-nkhnrki-c和c-rgmsrki-c，其中优势克隆序列为c-rrpaqsg-c；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。
• Assaying of the protective effect of mimotopes we divided the mimotopes into three groups based on their amino acid sequences " homology, with phage ml3 as the control group, then immunized 6-week balb / c mice, 10 mice per group . after amplifing positive phages and quantification, we used them to immunizing mice subcutaneously at 2-week intervals . the dose given is 100ug phage per mouse at first immunization and 30ug phage per mouse at the latter two immunizations
  并且在这12个阳性克隆中，有8个可特异性的与hini多抗结合，可能为二者共有的抗原表位口2.模拟表位的免疫保护作用分析根据模拟表位的序列相似性分3组免疫6周龄的雌性balb/c小鼠，10只/组，以野生噬菌体为阴性对照。
• Assaying of the protective effect of mimotopes we divided the mimotopes into three groups based on their amino acid sequences " homology, with phage ml3 as the control group, then immunized 6-week balb / c mice, 10 mice per group . after amplifing positive phages and quantification, we used them to immunizing mice subcutaneously at 2-week intervals . the dose given is 100ug phage per mouse at first immunization and 30ug phage per mouse at the latter two immunizations
  并且在这12个阳性克隆中，有8个可特异性的与hini多抗结合，可能为二者共有的抗原表位口2.模拟表位的免疫保护作用分析根据模拟表位的序列相似性分3组免疫6周龄的雌性balb/c小鼠，10只/组，以野生噬菌体为阴性对照。
• Recently, some new studies suggest that small cytokine peptide-mimics can function as the agonist or antagonist of cytokine receptor, and these small molecular peptides can be synthesized easily and oral adminstration with high concentration . based on these work, we have tried to set up experiment on tnfa-binding peptides and tnfa mimotopes which may be as leads of tnfa antagonist
  近年来的研究发现，小分子细胞因子模拟物具有细胞因子受体激动剂或拮抗剂的作用，且小分子模拟物因容易合成并能够给予高浓度、可口服等优点使其具有更加广阔的研发与应用前景。
• These phage display peptides may be the tnfa mimotopes . part iii specific c7c tnfa mimotope phage clone as target for screening of tnfa specific binding-peptides from 12mer phage display peptide library : by using the specific c7c tnfa mimotope as target, we developed a novel approach for screening phage peptide library targeting specific phage display peptides . we used c7c tnfa mimotope phage clone lcs-7 ( c-rrpaqsg-c ) as target to screen 12mer phage peptide library and identify the positive clones by phage elisa
  3、以特异性噬菌体克隆为靶筛选肽库的研究??一种肽库筛选新方法的建立：为了深入研究细胞因于与配基（受体或抗体）相互作用的关键残基及其空间构象，在上述研究基础上，我们以tnfa表位模拟肽克隆为靶筛选噬菌体十二肽库，找寻与之结合的tnfa结合肽噬菌体克隆；并以此拓宽了噬菌体肽库的应用范围。