Enzyme cutting analysis indicated that they had different enzyme cutting sites and cutting site number 利用常见的限制性内切酶酶切分析表明,二者有不同的酶切位点和切点数。
Dr . roberts current research is in bioinformatics and the discovery and mechanism of restriction - modification systems from bacteria Roberts博士目前的研究集中于生物信息学和细菌的内切
The deduced amino acid sequence of hau3r protein suggested that propablely it is a kind of endonuclease similar to ea59 根据其核苷酸序列推译的氨基酸序列暗示hau3 ~ r蛋白可能是一种类似于ea59的核酸内切酶。
To screen the library , defferential screening had been employed . sixty - four clones were randomly picked to perform dot blot 随机挑出13个克隆进行限制内切酶分析,结果有10个质粒含有300一600bp的插入片段。
3 . pbv220 - endostatin was transformed into e . coli dh5a . the positive colony was screened and identified by pcr and restriction endonuclease digestion 3 .将重组质粒转化到克隆菌dh5a中,经pcr筛选和限制性内切酶鉴定,得到阳性克隆菌株。
It was thought to be a new gene encoding for chitinanse of b . subtilis . genome library methodology was adopted for cloning of endoglucanase encoding genes 虽然同源性较低,但酶活性表达较强,认为该基因是编码内切葡聚糖酶的一个新基因片段。
" total eclipse begins " - when the moon touches the umbra internally , at this time , the moon is totally immersed in the umbra and total eclipse starts 当月球和本影内切时称为食既,这时月球全部进入本影,全食开始月球中心和地影中心距离最近时称为食甚
" total eclipse begins " - when the moon touches the umbra internally , at this time , the moon is totally immersed in the umbra and total eclipse starts 月球同本影接触时称为初亏,这时月偏食开始当月球和本影内切时称为食既,这时月球全部进入本影,全食开始
The genome could be digested only by restriction endonucleases hind , accl and xbal , and was resistant to cleavage by a wide range of other restriction endonucleases 病毒的基因组可以被hind 、 acc和xba酶切消化,但不能被其他所使用的限制性内切酶消化。
The orf of the hbrp coding a peptide of 233 aa , using the dnasis v2 . 5 demo analysis its structure and pi 3 . screening 1 ) screening using restriction enzyme Pcr产物和pgex一sx一1融合表达载体分别用bamhi和x五01限制性内切酶双酶切后连接,构建融合表达载体。