Therefore , it is important to study the profiles of gene expression , which will help understand the global cellular stress responses to chemical carcinogens , and further elucidate the mechanisms of nontargeted mutagenesis 所以，从整体上研究烷化剂作用后细胞基因表达谱改变，对于了解化学致癌物诱发的哺乳动物细胞应激反应的全貌和揭示非定标性突变的发生机制具有非常重要的意义。
The effects of sfamnpv on apoptosis - related protein bcl - 2 and cyto c were investigated by western blotting . sfamnpv could down - regulate the level of bcl - 2 expression after induction for 8h . also , cyto c was released from mitochondrial to cytosolic after induction for 4h 杂交可看到sl - 1细胞在接种病毒的4h后线粒体中的细胞色素c被释放到了细胞质中，这与很多在哺乳动物细胞中所作的结果是相同的。
Mammalian cells are among the best systems for biopharmaceutical production due to their excellence in post - translational modifications . the products they produced are much more similar to their natural forms than those produced by prokaryotic , yeast or insect cells 哺乳动物细胞表达系统具有准确的转录后修饰功能，表达的糖基化蛋白药物在分子结构、理化性质和生物学功能方面最接近天然蛋白分子，是目前重组糖蛋白药物生产的首选体系。
Ts87 gene fragment was picked out by screening adult t . s cdna library using sera of rabbit raised against soluble antigen of t . s and using sera of rabbit infected artificially with t . s . the ts87 fragment was ligated to vector pcdnas . l , which contains a cmv promoter / enhancer 本研究采用通过免疫筛库得到的旋毛虫抗原基因片段ts87与载体pcdna3 . 1连接。该载体含有人类巨细胞病毒( cmv )的高效启动子和增强子，是一种外源基因在哺乳动物细胞内高效表达的理想载体。
All different cyclins found in mammalian cells contain a 100 amino - acid homologous region named cyclin box , which is the molecular structure marker of cyclins . the expression level of cyclins shows cell cycle phase specialty . it can be regulated by transcription and the degree of protein hydrolysis 在哺乳动物细胞发现的12型细胞周期蛋白中均含有一段由100个左右氨基酸组成的保守区，称为周期蛋白盒( cyclinbox ) ，它是细胞周期蛋白的分子结构标志。
The previous studies in our laboratory have demonstrated that low concentration mnng treatment can induce nontargeted mutagensis in mammalian cells , and the mutagenesis is at least partly caused by the gene expression changes initiated with the activation of several signaling transduction pathways 我们实验室的研究证明低剂量mnng处理后的哺乳动物细胞中也存在非定标突变，并且哺乳动物细胞非定标突变的发生机制依赖于细胞信号转导通路介导的基因表达改变。
By immunizing rabbits with aa 192 - 326 fragment , polyclonal sera were obtained that were able to recognize e1 proteins expressed in both e . coli and mammalian cells , suggesting that e . coli - derived e2 proteins carried hcv e1 - specific , glycosylation - and - conformation - independent epitopes 利用aa192 - 326免疫家兔，获得了可识别大肠杆菌和哺乳动物细胞表达之e1蛋白的多抗血清，表明大肠杆菌系统表达的e1蛋白携带有hcve1特异的、不依赖于糖化和立体构象的抗原决定簇。
Found the phenomenon observed in the nematode caenorhabditis elegans for the first time in 1998 , consequently similar processes have been described for drosophila melanogaster , trypanosome , mammals including humans . the mechanism is that sirnas is the mediator , which can induce the risc to the target mrna and degrade it . recently there was great progress in the specific gene therapy and anti - virus , and rnai has been a focus of rna molecular therapy 自1998年fire等在研究线虫时首次发现以来，相继在果蝇、涡虫、锥虫、小鼠及哺乳动物细胞中发现rnai现象。一般认为： rnai第四军医大学硕士学位论文效应作用机制是sirnaskmallinterferinguaduplex ）作为中介分于，引导risc （ rnaiinducingsuppresscomplex ）至靶基因m洲a处，随后核酸内切酶将之降解。
We have previously established arsenic - resistant cells from cho cells and human lung adenocarcinoma cells ( cl3 ) . they are designated as sa7 and cl3r15 , respectively . we have recently demonstrated that arsenic extrusion in both sa7 and cl3r15 cells is mediated by an energy dependent and verapamil - and cyclosporin a - sensitive transportation system 哺乳动物细胞排砷机制之探讨：哺乳动物细胞如何将砷化物排除，至今仍未详加研究，本研究室曾筛选出抗砷的中国仓鼠卵巢细胞（ sa7 ）及人类肺腺癌细胞（ cl3r15 ） ，此两种抗砷细胞可能具有排砷帮浦可快速将砷排除。
