聚合酶链式反应

• A conservative motif , recognized by proteinases of potato virus y , was inserted between nib and ppiv , which will release functional ppiv from the fused protein after infection by potato virus y . then , plant expression vector pnpa was constructed by ligating the fusing gene and pbi121 , which is knocked out gus gene
  以马铃薯y病毒的复制酶( nib )基因为模板，通过聚合酶链式反应获得nib基因，并在nib基因末端保留了在病毒基因组中nib与外壳蛋白( cp )基因相衔接的保守序列。