In fact , it has been reported that mitochondrion shsp , cytosolic shsp and er shsp are related to the ability of cold - tolerance . but it does not know if the chloroplast shsp has the same pretective functions against cold sresses too 已有实验证明线粒体shsp 、内质网shsp 、细胞质shsp与细胞的耐寒性有关，而叶绿体shsp是否也与植物的耐寒性有关还不清楚。
There exists two isoforms of pges , glutathione - dependent membrane - associated pges ( mpges ) and glutathione - dependent cytosolic pges ( cpges ) . pges has been proved to be important for mouse implantation and decidualization , and for ovulation in bovine 根据pges在细胞内的定位和对谷胱甘肽的依赖性，分为膜相关的谷胱甘肽依赖型的pges ( mpges )和可溶性谷胱甘肽依赖的pges ( cpges ) 。
The effects of sfamnpv on apoptosis - related protein bcl - 2 and cyto c were investigated by western blotting . sfamnpv could down - regulate the level of bcl - 2 expression after induction for 8h . also , cyto c was released from mitochondrial to cytosolic after induction for 4h 杂交可看到sl - 1细胞在接种病毒的4h后线粒体中的细胞色素c被释放到了细胞质中，这与很多在哺乳动物细胞中所作的结果是相同的。
The results indicated that the content of cytosolic cam in cells treated with exogenous ca2 + has increased indistinctively , while fluorescence intensity in cells treated with tfp decreased . so we believed that exogenous ca2 + has little effect on the expression of cam 结果发现，经过外钙处理的酵母细胞胞内cam的表达量有所增加，但并不明显，因此认为增加外钙浓度对胞内cam的表达影响不大，但使处于活化态的cam相应增加。
There were little difference between gp mutant and control in the number of germinated pollen grain in vivo . the concentration of cytosolic free calcium in both pollen grain and pollen tube was lower in ga mutant and higher in ga overexpression strains than that of control 证实细胞中的游离钙离子参与了g蛋白调节的花粉萌发过程，即g蛋白对花粉萌发的调节有可能是进一步通过调控质膜钙通道和调控plc活性动员下游胞浆中钙离子水平来实现的。
Consequently , the measurement of cytosolic ca2 + has become an important area of investigation in biological and medical research . at present , most measurement of intracellular ca2 + are accomplished using fluorescent ca2 + indicators developed by tsien , such as quin - 2 , indo - l , fura - 2 and fluo - 3 因此，细胞游离钙含量及其动态分布变化的测定已成为化学、生物学和基础及临床医学研究中的一个非常重要的研究领域，亦是现代分析化学重要的前沿研究热点。
Rab gtpases are involved in cell differentiation , migration , endocytosis and exocytosis , cytosolic proteins transport and regulation of the transport and fusion of vesicle . in neuron cells , rab gtpases play a crucial role in synaptic vesicle transmission and transmitter release Rab蛋白主要通过调节囊泡( vesicle )的出芽( budding ) 、运输、锚定和融合，参与细胞的分化发育、细胞的内吞和外排、胞内蛋白的运输、交换和定位以及神经递质的运输和释放。
In this work , the transgenic plants showed a tendency to accumulate less na + and more k + under saline condition than wild type plants and there was a high cytosolic k + / na + ratio in the roots of transgenic lines . 5 . x - ray microanalysis of na + , k + contents of the symplast and apoplast showed that transgenic lines accumulate less na + and more k + in the symplast than wild type plants 在对转基因植株及野生型植株用100nunol lnaci处理六天后， na ” 、 k ”的x一射线微区分析，转基因植株根、叶中质外体na 、 k十含量变化不大，但共质体中na十含量明显降低，广含量高于对照。
Lovrin 10 and cv . 5389 . by using this system , following questions were investigated : the change of the pattern of microtubules when treated with iwf ; the change of cytosolic calcium levels in the protoplasts when treated with iwf and the influence of microtubule depolymerization prior to iwf treatment on the levels of cytosolic calcium 试验的目的是以激发子?原生质体简化试验系统来模拟叶锈菌侵染小麦叶片的互作体系，探讨激发子刺激后寄主植物微管骨架的动态变化及胞内ca ~ ( 2 + )水平的变化规律，为进一步探讨激发子诱导防卫反应的信号转导途径奠定基础。
The level of cytosolic free calcium in gp mutants is not different . adding exogenous purified cam to the cultural medium increased the pollen germination , tube growth , and cytosolic free calcium concertration in both wild type and ga mutant . however , the former was more sensitive to exogenous cam than the latter 外源钙调素(花椰菜和拟南芥11亚型， 10一7m )处理能够提高野生型及gpol缺失突变体中的游离钙离子水平，但对野生型花粉细胞游离钙离子水平的激活效应高于gpal缺失突变体。
