In addition , all of these indicators described above are difficult to target precisely to specific intracellular locations . when they are transfected into cells , they not only distributed in cytosolic compartment but also in the nucleus and this may cause some disaccuracy for real cytosolic ca2 + measurement 本文靶向性细胞ca ~ ( 2 + )荧光探针的设计合成及其在生命科学研究中的应用研究，使细胞钙的测定由单细胞水平拓展到了亚细胞水平，为更深入的细胞信号转导研究提供了可行、有效的手段。
At that time , cytosolic fluorescence intensity decreased to normal level , which shows that most of cells get through the gl / s point and enter the log phase . when cultured in medium that neucl was omitted , most of the cells were synchronized at gl stage of cell cycle . with flow cytometry , we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage 在激光扫描共聚焦显微镜下观察不同周期时相裂殖酵母细胞中cam的浓度及分布变化，结果表明，分裂期细胞总体荧光强度强于间期细胞；而对同一细胞内荧光强度的分析说明，间期细胞的荧光主要分布于胞质中，细胞核内则分布较少；而正在进行有丝分裂的细胞内荧光主要集中于赤道板处；刚完成有丝分裂的细胞内荧光则相对集中于两端或其中的一端。
5389 . in the protoplasts which mts were depolymerized by oryzalin prior to iwf treatment , the increase of cytosolic calcium was even more high that in the one which were not depolymerized . these results mean the depolymerization of mts caused 52 by the increasing influx of extracellular calcium 但将两个品种的原生质体预先用黄草消( oryzalin )解聚微管后，再以激发子刺激，两品种原生质体的胞钙浓度都较未解聚微管的对照有所增高，并且洛大林10原生质体中胞钙浓度的升高更为明显。
Having established certain conditions by culturing sl - 1 cells in grace medium with 7pm bapta / am and 110mm cacl ; , which sustained a cytosolic ca2 + clamp yet did not depleted intracellular ca2 + stores and induced apoptosis , the apoptosis in sfamnpv - infected sl - 1 cell was detected . the results showed the condition could not prevent apoptosis in sl - 1 cells . taken together , these data suggested that the depletion of er ca2 + stores rather than the elevation of cytosolic ca2 + or extracellular ca2 + entry contributed to sfamnpv - induced sl - 1 cells apoptosis 经细胞经流式分析和梯形带等定性及定量检测显示，两种处理条件均未能抑制sfamnpv诱导的sl一1细胞凋亡，即胞质内游离c扩十升高和胞外钙离子内流都不是诱导细胞凋亡的主要诱因，提示内质网钙库c扩明卜空是sfamnpv诱导的sl一1细胞凋亡信号转导通路中的主要媒介。
This hypothesis provide a new thinking on the action of steroid hormone on neurons , and is both a challenge and a supplement to the traditional genomic theory , which held that the action of steroid hormone is solely mediated by its intracellular cytosolic receptor within the cell nucleus 年代首次在国际上提出糖皮质激素作用于神经元的快速、非基因组机制或膜受体假说。这是对传统崽体激素基因机制或细胞学说的挑战与补充，受到国际学术界的高度评价，曾应邀在第
Furthermore , more and more evidence indicate that various rab proteins concertedly and concordantly regulate the cytosolic protein transport . at present , more than 40 rab gtpases were found , but according to the human genome analysis , it is estimated that about 60 rab ests exist totally Rab蛋白和另浙江大学硕士学位论文单核细胞系分化相关小g蛋白rab7b一种调节蛋白snare ( n一ethyl一maleimidesensitivefactor一solublensfattachmentprotein )在介导蛋白转运上可以说是一对功能互补的调节蛋白，只是侧重点不同。
In order to study the mechanism of the effect of low concentration tfp on the proliferation of s . pombe , we watch yeast cells loaded with fluo - 3 under laser scanning confocal microscope ( lscm ) . the fluorescence intensity reflected the cytosolic free calcium concentration . the result showed that , the cytosolic free ca2 + concentration in s . pombe cultured in ca2 + - free medium was 2 ~ 3 times lower than that in s . pombe cultured in medium containing 10umol / l ca24 , while ca2 + concentration in s . pombe treated with 50umol / l tfp was 4 - 5 times higher 本文发现增加胞外钙浓度以及低浓度( 20 100 mol l )三氟拉嗪( tfp )不但能促进野生型s . pombe细胞的增殖，而且对mfp7菌株也有同样的效应，这说明胞外ca ~ ( 2 + )和低浓度tfp对不同遗传型裂殖酵母细胞的增殖均有促进作用。
We used fission yeast schizosaccharomyces pombe ( s . pombe ) , an unicellular eukaryotic organism , as research material . electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ) , we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell . flow cytometry provided a way of determining the relative dna content of populations of fission yeast 本文以单细胞的真核模式生物裂殖酵母( schizosaccharomycespombe )为研究材料，通过激光扫描共聚焦显微镜观察酵母细胞胞质内游离ca ~ ( 2 + )的分布及相对浓度，以及不同周期时相细胞中gfp - cam的荧光强度变化，并采用细胞流式法对酵母细胞的相对dna含量进行测定以确定细胞所处周期时相。
Prostaglandin e2 ( pge2 ) is important for blastocyst spacing , implantation and decidualization in the rodent uterus . pge synthase ( pges ) catalyzes the isomerization of pgh2 to pge2 . there are at least three isoforms of pges , including glutathione - dependent and membrane - associated pges ( mpges ) , glutathione - independent and membrane - associated pges ( mpges - 2 ) , and glutathione - dependent cytosolic pges ( cpges ) 目前的研究表明至少存在三种不同类型的pges ，即膜相关谷胱甘肽依赖的pges ( mpges ) ，膜相关谷胱甘肽非依赖型的pges ( mpges - 2 )和可溶性谷胱甘肽依赖型pges ( cpges ) 。
The rab homologues in yeast all referred as ypt / sec4p , while in human they are named as rab . rabs are generally specifically localized to the cytosolic face of distinct intracellular membranes . in order to maintain the continuity of protein transport and the integrity of organelle membrane , an intracellular organelle may possess more than two rabs , e . g Rab蛋白的细胞内定位十分广泛，分布于胞内各个细胞器的胞膜浆侧，同一个细胞器可以有多个rab蛋白，以维持蛋白转运的连续性和细胞器膜的完整性，如晚期内吞体就同时拥有rab7和rab9 。
