Then the zona and polar body of oocytes treated with drug were removed by transferring into the m2 containing 0 . 5 % protease . the mouse fetal fibroblast cells were glued to the oocytes memb 该结果与重构胚的体外发育相符，表明正是这些己恢复微组装功能的化学去核卵母细胞才对其供体核具有重编程的支持能力。
Vessel owners try to protect their interests by engaging independent bunker surveyors to check and certify that the correct quantity and quality of fuel oil has been supplied 有些船主会聘请独立的燃油检测员去核证燃油的数量和质素，以保障自己的利益。可是，由于回佣丰厚，检测员经不住诱惑而同流合污，亦不足为奇。
Within 2h isolation from the follicle 100 % of oocytes underwent germinal vesicle breakdown and both the nucleolus and the nuclear envelope disappeared . after culturing for 6 h , prometaphase occurred in 90 % oocytes 本研究旨在探索一种崭新的、化学试剂诱导去核卵母细胞为核受体的、无透明带的、手工体细胞核移植方法。
In order to know if the genetic constitution of recipient oocytes influenced the development of embryos reconstructed from es cell , oocytes of c57bl / 6j and kunming albino were used as recipient , respectively 为了了解受体卵的遗传内涵是否对胚胎干细胞重构胚发育也会产生影响，分别以c57bl 6j和昆明白小鼠的m期去核卵为受体，构建胚胎干细胞重构胚。
After 15h of maturation . 98 % of oocytes released the first polar body and thus the first meiosis of mouse oocytes ended . secondly , strong chromosome to chromosome binding was induced by culturing pro - mi oocytes in demecolcine supplemented medium 第一次减数分裂期小鼠卵母细胞经化学去核后，去除透明带，并胎儿成纤维细胞粘合、电融合并srcl2激活，培养于微孔中。
Traditionally , somatic nuclear transfer in mammal is completed by micromanipulation . but micromanipulation is evidently damaging to cytoplast cytoskelton , causing the lost of some important factors involved in programming of donor karyoplast , reducing the volume of cytoplasm 传统核移植总效率低，且需要熟练的显微操作技术，所需设备昂贵，限制了核移植的广泛应用；因此作为替代，化学去核应运而生。
The results indicate that maturation rate of buffalo oocytes increase as the ivm duration prolongs , while the enucleation rate decreases , however , which has no significant difference on development of nt embryos with different enucleation time of oocytes during ivm16 ~ 24h 结果表明，水牛卵母细胞的成熟率随着成熟时间的延长不断提高，而去核率呈下降趋势，但对其随后的核移植效果没有显著影响(在ivm16 24h期间) 。
5 . the position of buffalo oocyte nucleolus related to its pb1 during the final stage of ivm , and the feasibility of increasing enucleation rate using spindle - view system were studied . the space between pbi and spindle ( sp ) of ivm buffalo oocytes increased as the ivm duration prolonged 9 ， 64刀） ，但当成熟时间增加到24h时，这一比例下降到57石（ 726 vs57石， p 0刀5 ） ，如采用spindle view系统去核贝可消除这种位置变化对去核率的影响。
The human fertilisation and embryology authority ( hfea ) has announced a full public consultation on licensing applications to investigate the potential of placing human genetic material into the ` empty ` shells of animal eggs for generating stem cell lines purely for research purposes 人类受精和胚胎学权威组织( hfea )近日公布了一份完整的公众调查，这份公众调查就移植人类遗传物质到动物去核卵母细胞产生纯粹用于研究的干细胞系的申请许可进行了可能性调查。
This study were designed to investigate development potency of the reconstructed embryos of black bear - cat inter - species nuclear transfer in vitro / vivo , to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm . somatic nuclear transplantation technology has been used to establish stem cell from different animals , which can provide materials to treat various diseases 本研究运用体细胞核移植的原理和技术，以六月龄雌性黑熊的成纤维细胞为供核体，去核未受精家猫成熟卵母细胞为受体，构建种间核移植重组胚胎，研究种问体细胞核移植胚胎的构建方法和重组胚胎的体内外发育潜能及其影响因素。
