In order to establish the technique of isolating es cell , and test the developmental ability of reconstructed embryos derived from es cell and oocytes of intra - or inter - species , mouse es cell were isolated from mouse blastocysts , and were used as donor nuclear , and were transferred into oocytes of various strains . the factors that influenced the development of reconstructed oocytes were determined . the developmental ability of embryos reconstructed with mouse es cells and rabbit oocytes was observed and analyzed 为了建立胚胎干细胞分离技术及了解其在同种和异种卵母细胞中的发育能力，首先从小鼠囊胚分离胚胎干细胞，并以它作为核供体，移植到同种不同品系去核的小鼠卵母细胞内，观察不同品系的卵母细胞对胚胎干细胞重构胚发育的影响。
Apple pieces apple dices ; cherries in syrup no stem , no stone ; cherries in syrup stem , whole ; baby - apples in syrup stem , whole ; canned mushroom ; fruit cocktails in syrup ; canned apple solid pack ; canned yellow peaches in syrup ; canned white peaches in syrup ; canned apricot in syrup ; canned grape in syrup ; canned pear halves in syrup 糖水酸樱桃去把,去核;蘑菇罐头;什锦水果罐头;干装苹果;糖水海棠带把,整粒;糖水黄桃罐头;糖水杏;糖水葡萄去核去皮;糖水洋梨;芦笋罐头;茄汁鲭鱼;烟熏贻贝
This study were designed to investigate development potency of the reconstructed embryos of rat - mouse and rat - bovine inter - species nuclear transfer in vitro / vivo , to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm . somatic nuclear transplantation technology has been used to establish stem cell from different animals , which can provide material to treat various diseases 本研究运用体细胞核移植的原理和技术，以成年大鼠的成纤维细胞为供体，去核小鼠和牛成熟卵母细胞为受体，构建种间核移植重组胚胎，研究种间体细胞核移植胚胎的构建方法和重组胚胎的体内外发育潜能及其影响因素。
By nuclear transferee transfered the nih 3t3 cell to enucleated gv and mii oocyte . the immunofluorescence results showed that jak . 2 was not transfered to somatoblast nuclear whether the recombined oocyte occured gvbd or not . when transfered somatoblast nuclear to enucleated and integrated m ii oocyte , the immunofluorescence results showed that jak2 only existed in the nuclear of integrated m ii oocyte but not the somatoblast nuclear . the nuclear transfer implied that the mature and immature oocyte cytoplasm could not transfer the jak . 2 protein to donor nuclear 通过核移植，将nih3t3体细胞核移入去核gv期和m期的卵母细胞中。荧光检测发现，体细胞核与去核gv期卵母细胞质构成的重组卵无论是否发生gvbd ，核内均没有jak2的出现。在去核和未去核的m期卵母细胞中移入体细胞核， jak2仅存在于未去核m期卵母细胞的核内，并不转移到体细胞核内。
The stage when the block occurs has been correlated to the time the embryonic genome takes over control of development from the maternal genome , which occurs in mouse at the 2 - cell stage . the embryos of balb / cxicr fl mouse show 2 - cell block in vitro and the effects of several culture media on overcoming the 2 - cell block in embryos development were studied . m16 ( sigma ) , in which 96 . 2 % of 1 - cell embryos developed to 4 - cell stage , were shown to be effective in overcoming the 2 - cell block 培养5h后， 53去核卵母细胞可见标记的mtocs ，但数量明显少于对照组，而又多于培养0h的去核细胞，说明此时53的化学去核卵胞质中确有mtocs的存在，其微管聚合能力正在恢复； mtocs标记，但其辐射产生的微管纤维短，结构还不够丰满；其余细胞的微管蛋白则丧失重新装配能力。
The company mainly produce various canned food , including canned grape in syrup , solid pack apple , cherries in syrup , yellow peaches in syrup , fruit cocktail in light syrup and apple in heavy syrup etc . . it been approved by ccib as a qualified export enterprise , the company has always pursued to strict processing technique , high quality and first - class management . the products has been enjoying a good reputation in japan , korea , australia , holland , malaysia , singapore etc . 公司生产的水果罐头海产品罐头冷冻蔬菜等20多个品种，投放市场后，受到国内外客商的高度评价，其中带把染色樱桃去皮去核葡萄草莓干装苹果什锦水果茄汁鱼原油鱼冷冻苹果丁及各种冷冻蔬菜等产品，出口到日本韩国澳大利亚荷兰马来西亚新加坡等国家，出口量占总产量的80 。
Since the success of dolly , the first cloned sheep with the adult somatic cells as karyoplast donor , new approaches have been developed for nuclear transfer technology . here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture . enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body 将培养10h的化学去核卵母细胞与供体成纤维细胞融合后lh 、 2h 、 3h ，分别有77 . 6 % 、 70 . 6 % 、 58 . 9 %重构胚的染色质发生凝集，其余胚胎的染色体则处于原核期;而只在融合后3h ， 27 . 9 %重构胚被标记出组装的纺锤体，且其中的同源染色体己经分离。
Sheep , mice , cattle , goats and pigs have been cloned , dolly - style , by transferring the nucleus of a cell from an adult animal into a hollowed - out egg . the success rate with cats was as low as with these animals . cc , born two months ago , was the only live birth from 87 cloned embryos that were implanted in surrogate mothers 至今为止，科学家们已经陆续成功克隆了绵羊老鼠牛山羊和猪，这些动物克隆的方式都和第一只克隆动物多利绵羊是相同的，即把成年动物细胞中含有遗传物质的细胞核植入去核的卵细胞中，并使两者融合，融合体分裂发育成克隆动物。
