The infected cells were passaged so that the virus could be isolated . cpe were observed from passage 2 to passage 9 postinfection . the cultured virus supernatants were harvested when cpe were observed in 3 / 4 infected cells 接种后的胚肺细胞从第二代到第九代均出现细胞病变，当接毒细胞有3 4出现细胞病变时，收集培养的细胞，制作超薄切片进行电镜观察，发现了病毒样粒子。
In py group , the mda level changes showed a equal based double peak , while in l2 and g group , the mda level changes showed a stepwise double peak , with the occurrence of a weak peak of mda at 1 h inoculation , and another higher peak at 6 h L2和g菌株可引起海带组织脂质过氧化双峰，第一阶段（接种后1h左右）速度快但强度弱，第二阶段（接种后6h左右）慢但强度较大， py菌株只引起海带组织中较微弱的脂质过氧化。
Research on envelope protein - based hcv vaccine demands a prompt solution to the problems of detection of antigen and evaluation of post - vaccination immune responses , which requires large amounts of highly purified envelope proteins and corresponding antibody or antiserum 基于被膜蛋白的hcv疫苗研究急需解决抗原检测和疫苗接种后的免疫反应评价的问题，需要首先得到大量高纯度的被膜蛋白抗原及其相应抗体或抗血清。
Results only 76 people with blood antibody titer > 1 : 5 had the cellular immune effect ( the particular plt was marc than 5 % ) , 35 people without antibody and 4 people whose blood antibody titer was 1 : 5 , nobody had the cellular immune effect 结果狂犬病疫苗接种后，抗体效价达到大于1 : 5的100人中， 76人同时产生细胞免疫力（即狂犬病毒特异性淋巴细胞转化率大于5 % ） ；而抗体效价等于1 : 5的4人和未产生抗体的35人中，无人产生细胞免疫力。
Degradation dynamics of the herbicide butachlor were investigated in nonrhizosphere soil , and various crops rhizosphere soils with or without inoculation treatment . the population dynamics of the cultureable microorganisms capable of utilizing butachlor as sole c and energy source were estimated with most probable number method in the process of butachlor degradation 本文考察主要作物棉花、小麦、玉米、水稻等作物根际土壤、接种后根际土壤和非根际土壤中除草剂丁草胺的降解速率，同时采用最大可能数( mpn )法测定分析丁草胺降解过程中丁草胺降解菌的变化动态。
There were a lot of gap - junctions between endothelial cells , smooth muscle cells and endothelial cells and smooth muscle cells . the number of the myoendothelial junctions had respect to the co - culturing time . after smooth muscle cells were seeded on the filter , the endothelial cells and smooth muscle cells can form the gap junctions through the micropores within 24 h 结果：共培养的ec呈单层生长，而smc呈多层生长，同类和异类细胞间都有缝隙连接形成，缝隙连接的形成与培养的时间有关，在smc接种后24h ， ec通过微孔与smc形成缝隙连接。
Two donkeys were injected intravenously with the pd70344v stock and developed acute diseases at 9 day and 12 day after injection , respectively . one animal ( donkey 03008 ) was euthanized due to the severity of the clinical symptoms on day 17 after injection and the other donkey ( 03009 ) died on day 18 after injection . we observed high fever , weigh lost , anorexia , depression , thrombocytopenia and high plasma viremia levels during the courses 两头驴（编号03008和03009 ）分别在接种后第9天和第14天体温升高40ac以上，稽留数日，产生急性病毒血症，其中一头于发热后期实施迫杀，另一头驴在第一次发热后自然死亡，病原学和病理学检查判定为急性马传贫。
The smg cells were subcutaneously injected into collagen sponge and the densities of cell suspensions were 10 - 70 10 / ml . we counted the number of cells and observed the histological changes with he staining , immunohistochem - ical staining and scanning electronic microscope after 1 , 3 , 5 , 7 days . 6 . the smg cells were subcutancusly injected into the scaffolds , whose porosity were 76 5 .将细胞浓度为( 10一70 ) x106 /血的领下腺细胞分别接种到胶原海绵支架上，复合培养;于接种后1 、 3 、 5 、 7d通过细胞计数、组织学、超微结构观察及免疫组织化学染色等方法检测何种接种细胞浓度为最佳。
6 ) after passage , the qinchuan - scalper skin fibroblast cells were in lag phase in first two day , then entered log phase and persisted six days , they stayed at plateur phase for about three days ; the mitotic index were most high at the fifth day after culture and toboggan at the sixth day 从生长曲线可以看出，以低糖dmem培养第4代秦川牛皮肤成纤维细胞，接种后滞留期缩短，约一天左右，之后为对数生长期，持续六天左右，进入平顶期。分裂指数最高时期在培养开始后的第五天，第六天急剧下降。
