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琼脂糖凝胶

"琼脂糖凝胶"的翻译和解释

例句与用法

  • Extensive mitochondrial dna polymorphisms were found among lagurus lagurus , mus musculu , rattus norvegicus and mice . the findings will help us to understand the dispersion and evolution of these animals
    通过琼脂糖凝胶电泳对这些片段进行测定,同时估算出草原兔尾鼠线粒体dna的长度约为16 . 6kb 。
  • 3 . to study physical and chemical property of dnaase the purified dnaase reacted with circular pbv220 - y - inf for an hour at 37 , then the producetion was detected by 1 % agrose gel
    双胸蚓组织中dna酶的酶学性质研究将纯化的dna酶分别与环状pbv220 - ? inf37反应1小时后,用1的琼脂糖凝胶电泳对其反应产物进行观察。
  • The 2nd pair of primers was designed according to the sequence of strain th - 98 collected in genbank . concentration of tp was analyzed after amplification invitro by rt - pcr , purified by low melt agarose and labeled by digoxigenin
    根据genbankth - 98株序列设计第2对引物,应用rt - pcr方法体外扩增该片段(命名为tp ) ,琼脂糖凝胶纯化后测定其浓度和纯度,进行非放射性地高辛标记。
  • Puried and identified by gel electrophoresis , the plasmid pbl29 is cccdna and the molecular weight of the plasmid pbl29 was estimated to be about 3 . 7kb . the sensitivity of bacillus iicheniformis29 to 18 antibiotics was determined by inhibitory zone with filter paper
    Licheniformis29菌株中抽提得到质粒pbl29 ,纯化后通过琼脂糖凝胶电泳观察,确证质粒pbl29为闭合环状质粒、分子量3 . 7kb左右。
  • In order to discuss the application of sol - gel in the preparation of biomembrane , the gel membrane of agarose was prepared by sol - gel with acticarbon and agarose as raw material , at the same time , the catalytic activity of immobilized cod based in the membrane was studied
    为探讨生物传感器用膜的制备,采用溶胶-凝胶法,以活性炭微粉和琼脂糖制备了琼脂糖凝胶薄膜并研究了薄膜固定cod的催化特性。
  • 2 . cloning of the pcr products the pcr products were purified by agarose gel electrophoresis and was ligated with pucm - t vector . by the method of pcr and enzyme digest analysis . the result shows that the plasmid containing cpti gene was transferred into e . coli dhso
    Pcr产物的克隆采用a / t克隆法,将pcr产物经琼脂糖凝胶电泳纯化回收后用t4连接酶与pucm - t载体连接,构建成克隆载体puc - cp ,转化大肠杆菌dh _ 5 。
  • A final period of extension was carried out for 9 min and final holding at 4 . the pcr products were resolved after electrophoresis in 2 % agarose gel with ethidium bromide . the pcr products were visualized while the gel was exposed to ultraviolet light
    扩增产物在2琼脂糖凝胶上电泳,用pcrmarker作分子量标准,紫外灯下观察,最适pcr反应条件为扩增出最强的sry产物带而无非特异性扩增带出现时的反应条件。
  • The high purity of genomic dna extracted by tripure isolation reagent was observed . dna agarose gel electrophoresis showed that the genome had a high integrity without degradation . and also , spectrophotometric analysis indicated that the genomic dna had no pollution by protein and rna . 2
    培养乳酸乳球菌nizor5 ,收集菌体,用tripurelsolationreagent提取其基因组dna ,琼脂糖凝胶电泳,紫外显示仪下可见在点样孔附近有一条整齐均一的dna带。
  • The apoptosis induced by extract of russula subnigricans hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis . the result showed that agarose gel electrophoresis of dna extracted from poisoned little white rat liver and kidney cells revealed typical 180 ~ 200bp integer - fold " ladder " " bands . apoptosis induced by extract of russula subnigricans hongo was dose - and time - dependentthe result indicated that extract of russula subnigricans hongo could induce apoptosis in little white rat liver and kidney cells
    4 .用电泳技术研究亚稀褶黑菇粗毒液诱导小白鼠肝肾细胞凋亡,小白鼠亚稀褶黑菇抽提液中毒后,肝肾细胞. dna经琼脂糖凝胶电泳出现180一200bp整数倍的ona梯形带, 19 . 09 / l一28 . 59 / l范围内,亚稀褶黑菇提取液诱导肝肾细胞凋亡表现出时间和剂量依赖性
  • Recovered the agarose and identified by agarose gelelectrophoresis . the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e . coli ( dh5a ) . the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
    Pcr产物经回收后,经琼脂糖凝胶电泳鉴定后,与pcambia1303连接并转化大肠杆菌dh5a ,阳性重组子经pcr和限制性内切酶酶切图谱分析,表明已获得海藻糖- 6 -磷酸合成酶基因的植物表达载体。
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