Extensive mitochondrial dna polymorphisms were found among lagurus lagurus , mus musculu ， rattus norvegicus and mice . the findings will help us to understand the dispersion and evolution of these animals 通过琼脂糖凝胶电泳对这些片段进行测定，同时估算出草原兔尾鼠线粒体dna的长度约为16 . 6kb 。
3 . to study physical and chemical property of dnaase the purified dnaase reacted with circular pbv220 - y - inf for an hour at 37 , then the producetion was detected by 1 % agrose gel 双胸蚓组织中dna酶的酶学性质研究将纯化的dna酶分别与环状pbv220 - ? inf37反应1小时后，用1的琼脂糖凝胶电泳对其反应产物进行观察。
Puried and identified by gel electrophoresis , the plasmid pbl29 is cccdna and the molecular weight of the plasmid pbl29 was estimated to be about 3 . 7kb . the sensitivity of bacillus iicheniformis29 to 18 antibiotics was determined by inhibitory zone with filter paper Licheniformis29菌株中抽提得到质粒pbl29 ，纯化后通过琼脂糖凝胶电泳观察，确证质粒pbl29为闭合环状质粒、分子量3 . 7kb左右。
2 . cloning of the pcr products the pcr products were purified by agarose gel electrophoresis and was ligated with pucm - t vector . by the method of pcr and enzyme digest analysis . the result shows that the plasmid containing cpti gene was transferred into e . coli dhso Pcr产物的克隆采用a / t克隆法，将pcr产物经琼脂糖凝胶电泳纯化回收后用t4连接酶与pucm - t载体连接，构建成克隆载体puc - cp ，转化大肠杆菌dh _ 5 。
The high purity of genomic dna extracted by tripure isolation reagent was observed . dna agarose gel electrophoresis showed that the genome had a high integrity without degradation . and also , spectrophotometric analysis indicated that the genomic dna had no pollution by protein and rna . 2 培养乳酸乳球菌nizor5 ，收集菌体，用tripurelsolationreagent提取其基因组dna ，琼脂糖凝胶电泳，紫外显示仪下可见在点样孔附近有一条整齐均一的dna带。
The apoptosis induced by extract of russula subnigricans hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis . the result showed that agarose gel electrophoresis of dna extracted from poisoned little white rat liver and kidney cells revealed typical 180 ~ 200bp integer - fold " ladder " " bands . apoptosis induced by extract of russula subnigricans hongo was dose - and time - dependentthe result indicated that extract of russula subnigricans hongo could induce apoptosis in little white rat liver and kidney cells 4 .用电泳技术研究亚稀褶黑菇粗毒液诱导小白鼠肝肾细胞凋亡，小白鼠亚稀褶黑菇抽提液中毒后，肝肾细胞. dna经琼脂糖凝胶电泳出现180一200bp整数倍的ona梯形带， 19 . 09 / l一28 . 59 / l范围内，亚稀褶黑菇提取液诱导肝肾细胞凋亡表现出时间和剂量依赖性
Recovered the agarose and identified by agarose gelelectrophoresis . the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e . coli ( dh5a ) . the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained Pcr产物经回收后，经琼脂糖凝胶电泳鉴定后，与pcambia1303连接并转化大肠杆菌dh5a ，阳性重组子经pcr和限制性内切酶酶切图谱分析，表明已获得海藻糖- 6 -磷酸合成酶基因的植物表达载体。
I and hindlll respectively . after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane , the southern blot was carried out using the cdna of rubber tree etrl as probe . the result of the southern blot showed that a hybridization band ( - 3 . 0kb ) turned up from the ecor i digested product and another band ( - 4 . 8kb ) turned up from the hindi ] 从橡胶树pr107品系的嫩叶提取基因组dna ，用限制性内切酶ecor 、 hinofll分别酶切，琼脂糖凝胶电泳分离并转膜后，用克隆的橡胶树etri基因的cdna片段作探针进行southern杂交分析，结果表明， ecor酶切在约3 okb处有一条杂交带出现， hi 。
To quantitatively measure the each cytokine levels from the spleens obtained before or after immunized mice , the total rna were extracted and generated into cdna by rt reaction . the native cdna was subjected into a competitive pcr reaction with a competitor that is a slightly larger in size 为了对免疫前后小鼠脾脏表达的细胞因子的水平进行定量检测，提取小鼠脾脏组织总rna ，经rt反应得到cdna ，利用一种修饰的竞争模板进行竞争pcr扩增，在琼脂糖凝胶电泳分析并与竞争模板产物比较而定量。
( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature . after 120d some cp cells died in the way of apoptosis . most nucleus of cells were condensed , and chromosome was marginated beside the nucleus inner membrane , cell sizes were reduced , dna ladder showed in dna gel electrophoresis ( 2 ) cp细胞系在10低温下细胞死亡率与时间成正比， 120d的细胞具典型的凋亡现象，即细胞核固缩、染色体边集在核膜内侧；细胞体积变小；琼脂糖凝胶电泳上显示特征性的“梯状”带。
