繁體版 English Francais
登录 注册

x-gal中文是什么意思

发音:  
用"x-gal"造句"x-gal"怎么读"x-gal" in a sentence

中文翻译手机手机版

  • 苷酶

例句与用法

  • Then the linked products were transformed into the high competent cell of e . coli dh5a . based on - complementation of the detective - galactosidase , positive recombinant clone were screened from x - gal plate
    从引物和基因序列的比对分析结果看, zhyf006序列与上下游引物的配对比例分别为s4
  • Fixed with gluteraldehyde , stained with ph 7 . 4 phosphate buffered x - gal dye solution , observed by reverse microscope , the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus
    病毒感染后的8h , 12h , 16h , 20h提取中肠组织,按常规石蜡包埋切片进行洗涤、透明、浸蜡、包埋、切片、粘片等操作程序,制成连续切片,在倒置显微镜下逐一进行观察计数。
  • After obvious cytopathogenic effects developed , virus - contained supematants were harvested , and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal . single blue plaques were picked , and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification . the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
    该载体与具有高度感染性的bartha - k61株基因组dna通过脂质体加plus法共转染vero细胞,采用甲基纤维素固定病变, x - gal染色,经过10代蓝斑纯化获得了一株稳定表达lacz基因的ge tk基因缺失突变株,命名为rprv - lacz 。
  • A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
    用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接;用连接产物转化大肠杆菌jml09感受态细胞,置含amp 、 x - gal和iptg的lb平板上培养12 20小时;挑取白色菌落于选择性培养基扩大培养,碱裂解法小量提取质粒dna ,并进行酶切分析鉴定,结果获得整合有prvge基因的重组质粒pugedna ,并与其它prv分离株进行ge基因序列同源性分析。
  • Thus the objective of this study is to find some ways to improve the efficiency of cell therapy , that is . to optimize the microenvironment of nscs and in turn prompt them to functinally repair abnormal cns . in the first part , optimization of x - gal staining method , which is correlated with ph , incubating time , perfusion and some other parameters , was successfully got and used in the subsequent experiment , viz , comparing the behaviours of primary nscs and immortal nsc line after transplantation
    鉴于此,为寻求各种方法提高移植效率,改善外源nscs存活及分化,进而促进其实现功能性替代,为临床nscs治疗提供理论基础,本实验进行了以下三部分的研究:实验第一部分研究了以lacz作为移植细胞标志基因时x - gal染色的影响因素,并得出排除非特异背景的优化条件。
用"x-gal"造句  

其他语种

x-gal的中文翻译,x-gal是什么意思,怎么用汉语翻译x-gal,x-gal的中文意思,x-gal的中文x-gal in Chinesex-gal的中文x-gal怎么读,发音,例句,用法和解释由查查在线词典提供,版权所有违者必究。