x-gal中文是什么意思
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用"x-gal"造句"x-gal"怎么读"x-gal" in a sentence
中文翻译手机版
- 苷酶
- "x"中文翻译 vt. (x-ed, x'd x-ing, x'ing) ...
- "gal"中文翻译 n. 【物理学】伽〔加速度单位〕。
- "gal" 中文翻译 : n. 〔俚语〕= girl. n. 【物理学】伽〔加速度单位〕。
- "b-gal" 中文翻译 : 苷酶
- "d-gal" 中文翻译 : d-半乳糖胺,d-氨基半乳糖
- "gal act" 中文翻译 : 半乳糖活化因子
- "gal aviadenovirus" 中文翻译 : 家禽禽腺病毒; 家禽禽腺簿
- "gal br" 中文翻译 : gallon]英制加仑; 英制加仑
- "gal circle" 中文翻译 : 辣妹掌门人
- "gal e" 中文翻译 : epimerase]半乳糖表异构酶; 半乳糖表异构酶
- "gal fridman" 中文翻译 : 贾勒弗里德曼
- "gal galena" 中文翻译 : 加利纳
- "gal galileo" 中文翻译 : 伽利略2
- "gal gallon" 中文翻译 : 加仑
- "gal givet" 中文翻译 : 盖尔吉韦; 贾尔・吉维特
- "gal hagar" 中文翻译 : 加勒哈加尔
- "gal in calico" 中文翻译 : 穿白色衣服的女孩
- "gal operon" 中文翻译 : 半乳糖操纵子
- "gal oya" 中文翻译 : 加尔奥耶; 加尔河
- "gal tardo" 中文翻译 : 加勒塔尔多
- "gal tt" 中文翻译 : 半乳糖耐量试验
- "gal vihara" 中文翻译 : 加尔寺
- "gal virus" 中文翻译 : 家禽病毒; 家禽簿,原鸡属腺簿样簿; 禽类腺病毒; 原鸡属腺病毒样病毒
- "gal zoltan" 中文翻译 : 加尔佐尔坦
- "gal(galileo)" 中文翻译 : 伽(重力加速度单位)
例句与用法
- Then the linked products were transformed into the high competent cell of e . coli dh5a . based on - complementation of the detective - galactosidase , positive recombinant clone were screened from x - gal plate
从引物和基因序列的比对分析结果看, zhyf006序列与上下游引物的配对比例分别为s4 - Fixed with gluteraldehyde , stained with ph 7 . 4 phosphate buffered x - gal dye solution , observed by reverse microscope , the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus
病毒感染后的8h , 12h , 16h , 20h提取中肠组织,按常规石蜡包埋切片进行洗涤、透明、浸蜡、包埋、切片、粘片等操作程序,制成连续切片,在倒置显微镜下逐一进行观察计数。 - After obvious cytopathogenic effects developed , virus - contained supematants were harvested , and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal . single blue plaques were picked , and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification . the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
该载体与具有高度感染性的bartha - k61株基因组dna通过脂质体加plus法共转染vero细胞,采用甲基纤维素固定病变, x - gal染色,经过10代蓝斑纯化获得了一株稳定表达lacz基因的ge tk基因缺失突变株,命名为rprv - lacz 。 - A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接;用连接产物转化大肠杆菌jml09感受态细胞,置含amp 、 x - gal和iptg的lb平板上培养12 20小时;挑取白色菌落于选择性培养基扩大培养,碱裂解法小量提取质粒dna ,并进行酶切分析鉴定,结果获得整合有prvge基因的重组质粒pugedna ,并与其它prv分离株进行ge基因序列同源性分析。 - Thus the objective of this study is to find some ways to improve the efficiency of cell therapy , that is . to optimize the microenvironment of nscs and in turn prompt them to functinally repair abnormal cns . in the first part , optimization of x - gal staining method , which is correlated with ph , incubating time , perfusion and some other parameters , was successfully got and used in the subsequent experiment , viz , comparing the behaviours of primary nscs and immortal nsc line after transplantation
鉴于此,为寻求各种方法提高移植效率,改善外源nscs存活及分化,进而促进其实现功能性替代,为临床nscs治疗提供理论基础,本实验进行了以下三部分的研究:实验第一部分研究了以lacz作为移植细胞标志基因时x - gal染色的影响因素,并得出排除非特异背景的优化条件。
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