angiostatic造句

• We used gradient dilution renaluration ( renaturation buffer : 0.14mol / l tris-hcl ph8.0 . 10mmol / lgsh, 2mmol / lgssg . 5 % glycerol, 0.02mol / l l-arg ) and gel filter renaturation by sephacral s200 to refold the inclusion body respectively . to get pure nk4, it was purified by gst affinity gromatochraphy . to detect the angiostatic activity of nk4 in vivo, it was tested using cam
  6)诱导温度对pg厂x一4t一l一nk4表达菌株bl21表达目的蛋自的影响分别进行了25’c、300c、37温度状态卜，以iptg化学诱导，发现其均可表达，全菌表达量:37最高，其次为30诱导时。