cleavaged造句

• Angiostatin ( as ) is a kind of polypeptide identified as a product of plasminogen cleavaged by protease . it blocks neovascularization by directly inhibiting endothelial cell proliferation and migration
  血管抑素(angiostatin，as)是血浆纤溶酶原(plasminogen，plg)经蛋白水解酶降解生成的多肽片段，是直接作用于血管内皮细胞的血管生成抑制剂。
• The sds-page results showed the fusion protein was efficiently expressed in the soluble form . 3 ) the expressed fusion protein was purified and cleavaged by enterokinase to release the mutation i of cmiv and the mutation ii, whiches exhibited antibacterial activity to the ecoli . k12
  三、对以上表达的融合蛋白进行初步纯化以及利用enterokinase切割融合蛋白，并进行抗菌活性检测，结果表明所设计的cmiv突变体具有抗菌活性。
• In this study, the recombinant plasmid pmd-18t-pea-h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet-28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet-28c-pea-h3 was constructed successfully . after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e . coli, the transformed hosts were induced by iptg, bysds-page and elisa analysis of host protein . the expression of the objective gene was detected, and it could account for 16.28 % of the total host protein . inclusion body was prepared from the incubating expression hosts induced by iptg
  同时将原核表达载体pet-28c用nco，xho双酶切，回收酶切产物，将回收的酶切产物pea，h3，载体进行连接，并转入dh5感受态细胞内，培养12-18小时后，挑取阳性菌落，经nco，xho双酶切分析及pcr检测，筛选到阳性克隆，其质粒测序结果表明成功地构建了毒性基因缺失的pea与人组蛋白h3融合基因的原核表达载体。