eaggec造句

• Schubert et al reported that hpi was found in 93 % eaggec, 27 % enteroinvasive e . coli, 5 % enteropathogenic e . coli and enterotoxigenic e . coli
  在不同类致泻性大肠杆菌中，hpi毒力岛在eaggec中高频分布的原因，目前也不清楚。
• The fragment containing mutant gene was subcloned into suicide vector pkng101, named pkh6, pkh 7 and pkh8 respectively . moreover, eaggec 17-2 was used to construct gene mutants by homologous recombination
  利用自杀质粒pkng101作为载体，构建了ip氏ip7和if’ps基因重组自杀质粒pkll6、pkh和吓。
• Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly . 7 eaggec hpi-positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi-negative did not so
  3n），将待测eaggec菌株的培养上清加入该报告菌株培养物中，用流式细胞术facs方法检测报告菌株荧光强度的变化情况，间接反映ybt的表达与否。
• Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly . 7 eaggec hpi-positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi-negative did not so
  3n），将待测eaggec菌株的培养上清加入该报告菌株培养物中，用流式细胞术facs方法检测报告菌株荧光强度的变化情况，间接反映ybt的表达与否。
• The reason that different distribution frequency of hpi in various e . coli is unclear, either . as the high frequency distribution of hpi in eaggec, this study will focus on the following parts to discuss the function of yersiniae high-pathogenicity island in enteroaggregative e . coli
  鉴于hpi在eaggec中的高频分布，本研究拟从eaggec菌株出发，着重从以下几个方面对eaggec携带的hpi毒力岛的功能进行了研究。
• Supernatants of eaggec grown under normal condition ( lb medium ) and iron deficiency condition ( led medium ) were applied to the reporter strain, wa-cs irpl : : kn ( pcjg3.3n ), which was transferred the reporter plasmid pcjg3.3n contained promoter region of fyua gene fused to gfp
  hpi毒力岛的力ua基因编码ybt受体fylla，该基因的表达受细胞外ybt水平的正向调节，本研究利用丹ua基回启动子融合表达报告质粒pcjg33n及菌株wacsirpl：kn（pcjg3
• 1 . the prevalence of hpi and functional expression of yersiniabactin in eaggec strains isolated from china using pcr methods, two eaggec type strains, eaggec 17-2 and eaggec o42, and 6 eaggec strains isolated from patients with diarrhea in china were investigated to detect hpi genes
  我国eaggec分离株中hpi的分布及表达耶尔森杆菌素功能的研究采用pcr扩增，对eaggec172禾eaggeco42tat准株及6株eaggec中国临床分离株进行了hpi功能核心区基因的分布检测。
• 1 . the prevalence of hpi and functional expression of yersiniabactin in eaggec strains isolated from china using pcr methods, two eaggec type strains, eaggec 17-2 and eaggec o42, and 6 eaggec strains isolated from patients with diarrhea in china were investigated to detect hpi genes
  我国eaggec分离株中hpi的分布及表达耶尔森杆菌素功能的研究采用pcr扩增，对eaggec172禾eaggeco42tat准株及6株eaggec中国临床分离株进行了hpi功能核心区基因的分布检测。
• 1 . the prevalence of hpi and functional expression of yersiniabactin in eaggec strains isolated from china using pcr methods, two eaggec type strains, eaggec 17-2 and eaggec o42, and 6 eaggec strains isolated from patients with diarrhea in china were investigated to detect hpi genes
  我国eaggec分离株中hpi的分布及表达耶尔森杆菌素功能的研究采用pcr扩增，对eaggec172禾eaggeco42tat准株及6株eaggec中国临床分离株进行了hpi功能核心区基因的分布检测。
• 1 . the prevalence of hpi and functional expression of yersiniabactin in eaggec strains isolated from china using pcr methods, two eaggec type strains, eaggec 17-2 and eaggec o42, and 6 eaggec strains isolated from patients with diarrhea in china were investigated to detect hpi genes
  我国eaggec分离株中hpi的分布及表达耶尔森杆菌素功能的研究采用pcr扩增，对eaggec172禾eaggeco42tat准株及6株eaggec中国临床分离株进行了hpi功能核心区基因的分布检测。
• 1 . the prevalence of hpi and functional expression of yersiniabactin in eaggec strains isolated from china using pcr methods, two eaggec type strains, eaggec 17-2 and eaggec o42, and 6 eaggec strains isolated from patients with diarrhea in china were investigated to detect hpi genes
  我国eaggec分离株中hpi的分布及表达耶尔森杆菌素功能的研究采用pcr扩增，对eaggec172禾eaggeco42tat准株及6株eaggec中国临床分离株进行了hpi功能核心区基因的分布检测。
• Using conjunction mobilization, we screened the mutant of eaggec 17-2 with the mutant phenotype by one step . compared mutant and eaggec 17-2, the experiments such as growth under iron-deficient conditions, virulence testing to the mouse, and ybt biosynthesis and regulation showed that ybtp, ybtq and ybtx genes have no significant effects on iron uptake, mouse virulen
  eaggec17毛携带的hpi毒力岛的功能研究eaggec172的ybtp、ybtqyybtx突变株没有表现出铁缺陷培养条件下的生长抑制表型，对ybt的合成无明显影响；对野生株与ybtp突变株的鼠毒力对比实验表明，lds。
• Using conjunction mobilization, we screened the mutant of eaggec 17-2 with the mutant phenotype by one step . compared mutant and eaggec 17-2, the experiments such as growth under iron-deficient conditions, virulence testing to the mouse, and ybt biosynthesis and regulation showed that ybtp, ybtq and ybtx genes have no significant effects on iron uptake, mouse virulen
  eaggec17毛携带的hpi毒力岛的功能研究eaggec172的ybtp、ybtqyybtx突变株没有表现出铁缺陷培养条件下的生长抑制表型，对ybt的合成无明显影响；对野生株与ybtp突变株的鼠毒力对比实验表明，lds。
• The results suggested that eaggec hpi-positive strains expressed the ybt system, but the presence of hpi core part does n't mean ybt expression . 2 . research on the function of ybtp, ybtq and ybtx genes in eaggec 17-2 ybtp, ybtq and ybtx genes from yen wa were cloned respectively and mutated, then inserted by a selectable substitution with chloramphenicokcaf ) gene in vitro
  携带hpi毒力岛的eaggec17毛ybtp、ybtq、ybtx基因功能研究克隆了小肠结肠炎耶尔森菌hpi毒力岛一rr7和叩结构基因，在体外进行精确突变后，插入氯霉素抗性基因（cat）作为选择性标记。
• The results suggested that eaggec hpi-positive strains expressed the ybt system, but the presence of hpi core part does n't mean ybt expression . 2 . research on the function of ybtp, ybtq and ybtx genes in eaggec 17-2 ybtp, ybtq and ybtx genes from yen wa were cloned respectively and mutated, then inserted by a selectable substitution with chloramphenicokcaf ) gene in vitro
  携带hpi毒力岛的eaggec17毛ybtp、ybtq、ybtx基因功能研究克隆了小肠结肠炎耶尔森菌hpi毒力岛一rr7和叩结构基因，在体外进行精确突变后，插入氯霉素抗性基因（cat）作为选择性标记。
• To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2-hybridized strains were re-confirmed and selected for this study . the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e . coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e . coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e . coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e . coli ( ehec ) and eaggec probe for entero-aggregative e . coli ( eaggec ) . the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e . coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e . coli o157 : h7
  为探讨携带小肠结肠炎耶尔森氏菌的hpi毒力岛的大肠杆菌是否具有其他已知的毒力基因，选取82株由原位杂交和pcr方法初筛irp2阳性的大肠杆菌菌株，进行在致泻性大肠杆菌的25个毒力基因的检测，包括肠产毒性大肠杆菌的热稳定毒素st和热不稳定毒素lt，肠侵袭性大肠杆菌的侵袭蛋白b基因ipab，肠致病性大肠杆菌的eaf、espc基因，肠出血性大肠杆菌的溶血素hly、志贺毒素1(slt1)、志贺毒素2(slt2)基因，肠集聚性大肠杆菌的eaggec探针，以及在泌尿道致病性大肠杆菌和o157：h7大肠杆菌中新发现的毒力岛基因。
• To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2-hybridized strains were re-confirmed and selected for this study . the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e . coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e . coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e . coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e . coli ( ehec ) and eaggec probe for entero-aggregative e . coli ( eaggec ) . the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e . coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e . coli o157 : h7
  为探讨携带小肠结肠炎耶尔森氏菌的hpi毒力岛的大肠杆菌是否具有其他已知的毒力基因，选取82株由原位杂交和pcr方法初筛irp2阳性的大肠杆菌菌株，进行在致泻性大肠杆菌的25个毒力基因的检测，包括肠产毒性大肠杆菌的热稳定毒素st和热不稳定毒素lt，肠侵袭性大肠杆菌的侵袭蛋白b基因ipab，肠致病性大肠杆菌的eaf、espc基因，肠出血性大肠杆菌的溶血素hly、志贺毒素1(slt1)、志贺毒素2(slt2)基因，肠集聚性大肠杆菌的eaggec探针，以及在泌尿道致病性大肠杆菌和o157：h7大肠杆菌中新发现的毒力岛基因。