After that we determined the presence of angiostatin gene in the putative recombinant virus with pcr analysis
The conserved cystein residues also present in the cub domain of clsp at position of 36 , 94 and 112
In pbivpt , it is under control of camv35s promoter . in pevp7 , it is under control of tomato - fruit - specific tfp promoter
Pcr ( polymerase chain reaction ) , southern blot analysis performed toward 10 transgenic rice plants revealed that foreign genes were integrated into the genome of these plants
The results showed that ybt - 803 contained cryia ( a ) , cryia ( b ) and cryia ( c ) genes , and ybt - 791 contained cryla ( b ) , cryla 拢 } and cryll genes . these genes were located in > 38mda plasmids
Armigera was analysed and then registered in genbank and accession number was af515667 . the cdna encodes a protein with a plltative 2l amino - acid signal peptide and a 425 amino - acid mature protein with molecular mass of 50 kda
The genomics dna of the transformants was extracted and assayed by pcr with nptii primer camv35 / cp primer and the results indicated that the chloroplast shsp gene has been integrated into the genomics of the tomato . then the transgenic tomato were exposed to low temperature ( in winter , on natural condition , the top temperature was 15 ? and the lowest temperature was 5 and a set of physiology parameters were measured after 6 weeks . the results were shown as follows : 1 ) effect on growth height of the transgenic tomato and the control plants after 6 weeks at low temperature showed that the transformants had been grown faster than the control . in addition , the leaves of the control plants appeared to be much reder than the transgenic tomato , and the change were obvious followed by far from the treated time at low temperature , which suggested that the constituently expression of the chloroplast shsp had some protective fountions to the tomato at low temperature
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