perfusing造句

• Adequate quantities of plasma continued to perfuse the kidney .
  相当量的血浆持续灌注到肾脏。
• The heart was perfused with a glutin solution of 30 % concentration . after that it was embedded with two steps to guarantee its anatomical position
  以30%浓度的明胶溶液灌注心脏，分两步进行包埋，以保证心脏处于解剖位。
• Amiodarone prevent electrophysiological changes of acute atrial dilation accompanied with rapid atrial stimulation in isolated langendorff-perfused rabbit heart
  离体兔心脏急性双心房扩张合并快速心房刺激时电生理变化及胺碘酮的干预作用
• Tacho y, pappus t, lauffenburger m, et al . cgrp stimulates somatostat in release from isolated perfused rat stomach j . endocrinology, 1986, 118 : 2144
  雷银雪，李兆申，湛先保，等.降钙素基因相关肽在大鼠应激性溃疡发生中的作用j.第二军医大学学报，1998,19(5):464-466
• The isolated hepatocytes, isolated perfused organs, tissue slices, liver cell lines, subcellular fractions and the genetically engineered cells are the most commonly used in vitro liver models nowadays, especially the isolated hepatocytes
  目前，常用的肝脏体外模型包括原代肝细模型、离体肝脏模型、肝脏切片模型、肝细胞系模型、亚细模型及基因工程细模型等，其中原代肝细型最?常用。
• D ) pain and electroacupuncture stimulated group : after the injection of 200ul formaldehyde ( 5 % ) in the right hind paw, the rats are stimulated with electroacupuncture immediately; the four groups of rats were perfused . sections were cut at 40um for the method of nadph-d
  疼痛刺激加电针刺激组，在大鼠右侧后脚掌注射5甲醛200ill后立即固定，并用针灸治疗仪刺激同侧后肢“足三里”穴3v5shz，持续0
• It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback ( mef ) . in the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts . controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts . thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level
  在心脏机械电反馈的研究中准确控制机械刺激是非常重要的.本研究室构建了一套适用于离体家兔心脏的心室压力钳系统.该系统通过计算机控制压力钳,不仅能模拟正常生理条件下左心室的压力波形,还能在心室活动周期的特定时相、以适当波形对心室施加机械刺激.该系统集心脏灌流与起搏、表面心电图记录、单相动作电位记录、心室压力钳制与测定等多种功能于一体,特别适用于器官水平上观察机械电反馈现象并探讨其机制
• It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback ( mef ) . in the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts . controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts . thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level
  在心脏机械电反馈的研究中准确控制机械刺激是非常重要的.本研究室构建了一套适用于离体家兔心脏的心室压力钳系统.该系统通过计算机控制压力钳,不仅能模拟正常生理条件下左心室的压力波形,还能在心室活动周期的特定时相、以适当波形对心室施加机械刺激.该系统集心脏灌流与起搏、表面心电图记录、单相动作电位记录、心室压力钳制与测定等多种功能于一体,特别适用于器官水平上观察机械电反馈现象并探讨其机制
• It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback ( mef ) . in the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts . controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts . thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level
  在心脏机械电反馈的研究中准确控制机械刺激是非常重要的.本研究室构建了一套适用于离体家兔心脏的心室压力钳系统.该系统通过计算机控制压力钳,不仅能模拟正常生理条件下左心室的压力波形,还能在心室活动周期的特定时相、以适当波形对心室施加机械刺激.该系统集心脏灌流与起搏、表面心电图记录、单相动作电位记录、心室压力钳制与测定等多种功能于一体,特别适用于器官水平上观察机械电反馈现象并探讨其机制
• Abstract : it is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback ( mef ) . in the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts . controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts . thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level
  文摘:在心脏机械电反馈的研究中准确控制机械刺激是非常重要的.本研究室构建了一套适用于离体家兔心脏的心室压力钳系统.该系统通过计算机控制压力钳,不仅能模拟正常生理条件下左心室的压力波形,还能在心室活动周期的特定时相、以适当波形对心室施加机械刺激.该系统集心脏灌流与起搏、表面心电图记录、单相动作电位记录、心室压力钳制与测定等多种功能于一体,特别适用于器官水平上观察机械电反馈现象并探讨其机制
• Abstract : it is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback ( mef ) . in the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts . controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts . thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level
  文摘:在心脏机械电反馈的研究中准确控制机械刺激是非常重要的.本研究室构建了一套适用于离体家兔心脏的心室压力钳系统.该系统通过计算机控制压力钳,不仅能模拟正常生理条件下左心室的压力波形,还能在心室活动周期的特定时相、以适当波形对心室施加机械刺激.该系统集心脏灌流与起搏、表面心电图记录、单相动作电位记录、心室压力钳制与测定等多种功能于一体,特别适用于器官水平上观察机械电反馈现象并探讨其机制
• Abstract : it is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback ( mef ) . in the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts . controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts . thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level
  文摘:在心脏机械电反馈的研究中准确控制机械刺激是非常重要的.本研究室构建了一套适用于离体家兔心脏的心室压力钳系统.该系统通过计算机控制压力钳,不仅能模拟正常生理条件下左心室的压力波形,还能在心室活动周期的特定时相、以适当波形对心室施加机械刺激.该系统集心脏灌流与起搏、表面心电图记录、单相动作电位记录、心室压力钳制与测定等多种功能于一体,特别适用于器官水平上观察机械电反馈现象并探讨其机制
• Half animals were perfused with paraformaldehyte following normal serum after test innnediatly, then removed the brain and fixed for 24 hours with paraformaldehyte, paraffin sections were prepared at 3um, histochemical staining for counting ce11 pro1iferating ratio and in situ hybridization for calculating integra1 scores of stem cell factor inrn positive signals
  结果如下：1增殖细胞的观察经72h的快眼动睡眠剥夺，实验组大鼠海马齿状回区brdu与p皿a（proliferatingcellneuclearantigen，pma）阳性标记细胞数显著增加。
• Objective : to establish a rat model of orthotopic gastric isotransplantation by using microsurgical techniques . methods : 70 sd rats were used in our experiment and 35 gastric trasplantations were carried out . in the donor's operation : after the spleen was resected and the proper liver artery was ligated, the stomach was perfused through the abdominal aorta . then the stomach was resected with its peripheral blood vessels including celiac trunk and the portal vein, etc . in the recipient operation : after the stomach and the spleen were reseeted, the implantation was performed by the following sequence : the end-to-side anastomosis between the portal veins . the end-to-end anastomosis between the celiac trunk and the left gastric artery . open the blood flow to observe the effect of the blood supply of the stomach . the end-to-end anastomosis between the duodenum . the end-to-end anastomosis between the cardiac and the esophagus . results : 35transplantations were carried out in which the operation success rate in the last 20 cases was 80 ( 16 / 20 ) . the average operation time was 2.35 h . the longest survival time was over three months . conclusions : the model of orthotopic gastric transplantation in rat was successfully established . it could be used to study the transplanted stomach in the abdominal multiviseeral transplantation and the reconstruction after the total gastrectomy
  目的应用显微外科技术,建立大鼠原位异体胃移植模型.方法70只sd大鼠,行35例次的胃移值手术.供体手术,先切除脾脏,经腹主动脉行原位胃冷灌洗.将胃及其所属血管,包括腹腔干和门静脉干等一并切取.受体手术,先切除胃和脾脏,分别行供、受体间门静脉的端侧吻合,供体腹腔干与受体胃左动脉的端端吻合,然后开放血流.再行供体和受体十二指肠间端端吻合,贲门与食管端端吻合.结果在施行的35例手术,后20例中有16例成功,成功率为80.最长存活者达3个月.结论成功地建立了大鼠原位异体胃移植类型.该模型可用于腹部多脏器移植中移植胃的相关研究及全胃切除术后代胃的研究
• Material and methods normal rats of male sd were divided into young, adult, and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0.5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50-100 ml and then 4 % paraformaldehyde pbs 100-400ml were perfused through the left ventricle of the heart, the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration, dipping into paraffin for embeding, and reshaping the pieces
  2）磷酸缓冲液100400m，30分钟灌注完毕，取出整脑，在上述固定剂oc）内后固定12小时。切取观察部位脑块，然后，进行梯度酒精脱水，浸蜡，包埋，修块，石蜡连续切片（德国leica石蜡切片机人切片厚度still，zlllll，蛋自甘油载片捞片，60c烤箱过夜，二甲苯脱蜡，梯度酒精置换，浸水，h六染色，梯度酒精脱水，二甲苯透明，中性树脂封片。室温风干后，显微镜观片，olympus万能显微镜照相。