t-dna造句

• A single step transformation system for the generation of a large - scale t - dna insertional mutant population of rice was developed
  基于水稻大规模t - dna插入突变体库的建立，我们发展了一个简单、快速、高效的农杆菌介导的一步转化系统。
• Insertional mutagenesis using t - dna , transposons and plasmids axe commonly methods to creating a mutant library . here we summarize these strategies as well as the progress in the functional genomes research
  本文分别介绍三种方法的原理及其在功能基因组学研究中的应用和研究进展。
• Pcr analysis of resistant seedlings with nptii gene primers showed that 6 out of 12 seedlings detected had the 700bp fragment specific to the plasmid pig121 , indicating that t - dna had been integrated into the genome of sweet cherry
  L ，负压的适宜处理是lmmxio次。 6 、通过p r扩增，初步证明证明外源基回己转入甜樱桃。
• Totally 99 transgenic rice plants from 125 resistant calli of 191 calli were obtained and pcr assay showed that 80 . 3 % of them were positive . the result of southern blotting analysis for primary plants revealed that each transgenic plant contained a average of 2 . 5 copy of t - dna
  对抗性植株进行pcr扩增检测，结果表明有80 . 3的抗性植株为阳性植株； southern杂交结果进一步证明了质粒的t - dna已经整合到水稻的基因组中，拷贝数为1 4个，平均每棵转化植株有2 . 5个拷贝。
• 2 . an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region
  以增强的ta29启动子驱动克隆的coda基因，构建成花药特异性嵌合基因表达盒；将此表达盒插入双元载体p3301 ，构建成以ppt抗性基因为选择标记，以gus为报告基因的植物表达载体。