vp4造句

• Vp4 and vp7 typing for rotaviruses identified from stool specimens collected from infants and young children with acute diarrhea in lanzhou
  2005年度婴幼儿病毒性腹泻的病原学研究
• Series of dna vaccines including pci - vp2 / vp4 / vp3 , pcdna3 - vp2 / vp4 / vp3 , pci - vp2 , pcdna3 - vp2 of ibdv strains zj2000 and jd1 were developed using immune - stimulating complexes ( iscom ) as the adjuvant
  33 ；弱毒苗b87虽对bc6 85株具有良好的保护，但对zj991株、 zj2000株的攻击不理想，平均保护率仅达60 。
• Consequently , of the 42 unique rotavirus strains identified to date based on their combinations of vp7 and vp4 varieties , only four or five account for more than 90 percent of rotavirus disease worldwide
  因此，根据不同种类的vp7及vp4蛋白组合，目前已?定出42种轮状病毒品系，但其中4 ~ 5种，就造成全球感染轮状病毒病例的90 %以上。
• At the same time , vp4 gene was mutated in a certain point by pcr . the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly . a . tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l . guad according to the leaf disc procedure
  为便于基因操作，对外壳抗原蛋白vp4基因进行适当的修饰和改造：通过引物设计，利用pcr反应，在基因的起始编码前引入有助于真核生物表达的kozak序列和限制性内切酶位点；使用套叠pcr对vp4基因进行定点突变，以便于将改造后的基因插入pbi121构建植物表达载体，通过直接转化法，把pbi121 vp4转入农杆菌eha105 ，构建了农杆菌工程菌。
• Transformed shoots were selected on solidified medium containing kanamycin . ten kanamycin resistant transformants were obtained by direct or induction calla . these transformants were checked by pcr , pcr - southern blot and southern blot , confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad
  对所得到的抗性转化株进行了pcr 、 pcr - southernblot 、 southernblot检测，其中2株呈阳性，证明vp4基因已整合到苎麻基因组dna中。