dna的pcr扩增造句

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dna的pcr扩增 DNA的PCR扩增是根据DNA变性，复性原理设计的，变性与复性的简介如下。从SDS、CTAB、SDS改良法1、SDS改良法2、CTAB改良法等5种方法中选择了SDS改良法2作为三色堇适合的DNA提取方法，同时构建了三色堇DNA的PCR扩增程序，并从Taq酶用量、dNTP浓度、引物浓度、DNA模板用量等方面优化了PCR扩增程序。
9700型pcr扩增仪 9700型PCR扩增仪用于医学实验，每个PCR方法运行时其时间和温度程序会实时动态显示，最多可储存100个完整的PCR方法。
pcr扩增荧光域值设定在PCR扩增的指数期。作为引物，对基因组DNA进行PCR扩增。 PCR扩增DNA片段只是一个重要手段。，然后加入5SrRNA引物PCR扩增38个循环。配对的PCR扩增进行实时监测。用分光光度计测定DNA浓度后进行PCR扩增。循环数决定PCR扩增的产量。这种结合是PCR扩增的基础。 RT-PCR扩增效能依赖于样本模板RNA的质与量。用PCR扩增CGG...
pcr基因扩增仪 PCR基因扩增仪血液流变学检测仪尿项分析仪经颅多普勒超声诊断仪心电图仪脑电图仪等。全自动生化分析仪PCR基因扩增仪特种光前列腺治疗仪高分辨率B超HW-IA微波治疗仪全自动精子分析仪等。拥有彩超、大型X光机、呼吸机、监护仪、美国罗氏PCR基因扩增仪、罗氏血气分析仪、全自动生化分析仪、血液透析机、人工肝支持系统等先进的诊疗设备。重点学科实验室设有基因诊断免疫学、高效液相、...
dna扩增置DNA扩增仪进行40次循环扩增。理论上循环20周期可使DNA扩增2n，即100余万倍。率先引进DNA扩增技术和流式细胞分析技术。（PCR）的DNA扩增技术目前已引入钩体病的诊断领域。定义：一种测定DNA序列中单碱基突变的DNA扩增技术。同样可采用DNA重组、DNA扩增酶联聚合反应（PCR）等新技术。简易扩增法的开发，大大拓宽了DNA扩增法的使用范围。主要包括...
dna dependent dna polymerase Examination of the " pol " genes that encode the DNA dependent DNA polymerase in various groups of viruses suggests a number of possible evolutionary relationships.
dna-dna hybridization The 7 isolates were identified based on physiological and biochemical characterization , 16s rdna sequence , g + c mol % and dna - dna hybridization . isolate tl was identified as bacillus...
随机扩增多态dna 5、丁鱼岁不同群体间形态学差异比较与随机扩增多态DNA（RAPD）。 18．四川大学学报（自然科学版），宽叶韭种内分化的随机扩增多态DNA分析,2002，39（5）：943－947，第二作者。
5700型实时荧光定量pcr仪 5700型实时荧光定量PCR仪是特异性靶基因检测与定量的一体化平台，用于医学实验。
allele specific pcr Determination of haplotypes can be done through molecular methods ( Allele Specific PCR, Somatic cell hybrids ). For a smaller number of SNPs, a uniplex assay like KASP ( Kompetitive Allel...
alu pcr Alu PCR is a " DNA fingerprinting " technique. Alu element can be used for genome fingerprinting based on PCR, which is also called Alu PCR.
anchor pcr The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr , and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anc...
anchored pcr RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5'end ( 5'RACE-PCR ) or 3'end ( 3'RACE-PCR ) of the RNA . This technique is some...
applications of pcr By default, a paper by Saiki and other scientists on the applications of PCR was published first. Many online tools are freely available for primer design, some of which focus on specific ...
arbitrarily primed pcr An experiment by Zhong et al . ( 2001 ) with arsenite-exposed human lung A549, kidney UOK123, UOK109 and UOK121 cells isolated eight different DNA fragments by methylation-sensitive arbitr...
colony pcr In the case of deletions, a single transformation protocol is more efficient and shows a 100 % deletion rate following colony PCR . Further work should be pursued to determine causes for t...
competitive pcr To quantitatively measure the each cytokine levels from the spleens obtained before or after immunized mice , the total rna were extracted and generated into cdna by rt reaction . the nati...
degenerate pcr By using inverted microscope , it was observed that dunaliella salina of different growth stages after the high osmotic shocks can live in the medium with nacl concentration between 0 . 1m...
digital pcr Digital microfluidics has been described as a means for carrying out Digital PCR. In 2006 Fluidigm introduced the first commercial system for digital PCR based on integrated fluidic circui...
electronic pcr The free Electronic PCR ) may be performed to assist in primer design. Computer simulations of theoretical PCR results ( Electronic PCR ) may be performed to assist in primer design.
error prone pcr The starting gene can be mutagenised by random point mutations ( by chemical mutagens or error prone PCR ) and proportion of functional variants it contains.
hot start pcr Hot Start PCR significantly reduces nonspecific priming, the formation of primer dimers, and often, increases product yields.
in situ pcr Analyzing 40 full-term placental tissues with immunohistochemical staining and RT in situ PCR, shows strong expression of synctin-1 in synctiotrophoblasts compared to cytotrophoblasts.
inverse pcr Inverse PCR is especially useful for the determination of compared with DNA databases to locate the sequence which has been disrupted. The inverse PCR method involves a series of restricti...
kompetitive allele specific pcr For a smaller number of SNPs, a uniplex assay like KASP ( Kompetitive Allele Specific PCR ) are used.