原核载体的英文

• prokaryotic vector

• "原"英文翻译    primary; original; former
• "载体"英文翻译    carrier; supporter; isotopic ...
• "真核载体" 英文翻译 :    eukaryotic vector
• "原核" 英文翻译 :    germinal nucleus; primary nucleus; prime kernel; procaryon; prokaryon; prokaryota; prokaryote; pronucleus; protocaryon; protokaryon
• "精原核, 雄原核" 英文翻译 :    male pronucleus
• "精原核,雄原核" 英文翻译 :    pronucleus male
• "卵原核, 雌原核" 英文翻译 :    female pronucleus
• "载体" 英文翻译 :    [化学] carrier; supporter; isotopic carrier
• "雌原核" 英文翻译 :    female pronucleus
• "精原核" 英文翻译 :    malepronucleus
• "雄原核" 英文翻译 :    male pronucleus; malepronucleus
• "原核的" 英文翻译 :    prokaryotic
• "原核素" 英文翻译 :    protonuclein
• "雌性原核" 英文翻译 :    thelyblast; thelykaryon
• "精子原核" 英文翻译 :    sperm pronucleus
• "静止原核" 英文翻译 :    stationary pronucleus
• "胚乳原核" 英文翻译 :    definitive nucleus
• "雄原核,雄前核" 英文翻译 :    male-pronucleus
• "与原核类的" 英文翻译 :    goldberg-hogness
• "原核期移植" 英文翻译 :    prost pronuclei stage transfer
• "原核生物" 英文翻译 :    procaryote; prokaryote; prokaryotic organism; protokaryotic
• "原核生物的" 英文翻译 :    procaryotic; prokaryotic
• "原核生物界" 英文翻译 :    kingdom monera; prokaryota; prokaryote
• "原核生物域" 英文翻译 :    prokaryote
• "原核微生物" 英文翻译 :    prokaryotic micro-organisms

• A 1 . 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites . the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge . strain dh5a of e . coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so
  以质粒ppgedna为模板， pcr扩增出1 . 7kb的ge基因完整片段，将扩增产物以ecori和bamhi双酶切后，插入原核表达载体pbv220的p _ rp _ l启动子下游的ecori和bamhi位点间，得到重组表达质粒pbvge ，转化了pbvge的大肠杆菌dh5a经温敏诱导表达后，用sds - page和western - blot ，以及琼脂双扩散来检测，结果表明prvfa株ge基因在原核载体上得到高效表达，表达产物约占总蛋白的17 。