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密度梯度离心的英文

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"密度梯度离心"怎么读用"密度梯度离心"造句

英文翻译手机手机版

  • density gradient centrifugation
  • density gradientcentrifugation
  • density gradine centrifugation
  • gradient centrifugation density
  • isodensity centrifugation

例句与用法

  • Purification : density gradient centrifugation ( sucrose & cscl )
    分离:密度梯度离心法(蔗糖&氯化铯)
  • Density gradient centrifugation
    密度梯度离心
  • The fetal liver stem cells were isolated by collagenase digestion , gravity sedimentation and density gradient centrifugation , identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition
    采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞,通过免疫细胞化学方法对其进行初步鉴定,以及应用流式细胞仪等对其生长状况进行评估。
  • The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
    本研究设计以c tamrna为模板的反义cdna片段,从c ta基因5 ’端第94位到500位核苷酸段设计引物,目的片段407bp ,覆盖第116和188位两个aug密码子,也包含了第一外显子和第二外显子间的剪接位点:用常规分子生物学方法构建了反义片段的腺病毒表达载体( padeasy - 1系统) ;腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒,用氯化铯密度梯度离心法获得纯化的高滴度腺病毒;进行体外基因转移,分别用反义片段真核表达载体转染p388d1细胞和用重组腺病毒感染hela细胞,观察导入的c ta基因反义rna抑制细胞内组成型或诱导型c ta基因表达的作用,从而达到调控mhc -类分子表达的目的。
  • Cell morphology was similar no matter what method was used . percentage of live cells was significantly higher in erythrocyte splitting method that was ( 91 4 ) % than in centrifugation method that was ( 83 5 ) % . proliferative ability of mscs seperated by erythrocyte splitting method at 2 to 6 generation was much higher than by another method . the maximal cell number was 4 . 67 vs 4 . 10 times that of the initial cell number at cell inoculation
    红细胞裂解法较密度梯度离心法细胞贴壁较慢, 36小时才可见零星细胞贴壁,两种方法细胞形态相似。活细胞百分比,红细胞裂解法为( gi士4 ) ,密度梯度离心治( 83士5 ) ,有明显差异。传代培养: 2 6代范围内,红细胞裂解法较梯度离心法细胞增殖更旺盛(分别是按种时细胞数的4
  • When the approach to get a direct binding molecule was proved not fruitful , attention was turned to find binding sources for the purpose of further isolating the binding protein . the first considered and also the relatively easier - obtained materials are some adhesion molecules . results showed , only n - cam 140 showed ambiguous binding
    , idin砰proteina磁珠为结合反应载体,从小鼠成年全脑匀浆作为结合起始材料,以蔗糖密度梯度离心法进4行细胞亚组分分离,以sds page显示阳性结合条带,以westernblot确定蛋白身份。
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