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无血清培养的英文

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"无血清培养"怎么读用"无血清培养"造句

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  • serum-free culture

例句与用法

  • Experimental condition optimization study on inducing mouse embryonic stem cells differentiation into insulin producing cells by serum - free culture in vitro
    无血清培养法诱导小鼠胚胎干细胞分化为胰岛素分泌细胞的实验条件优化选择
  • Objective to investigate the culturing technique for conjunctival epithelial cells and to evaluate the facibility of serum - free culture system on culturing conjunctival epithelial explants
    摘要目的研究结膜上皮细胞体外培养的方法,并进一步探索无血清培养系统用于结膜上皮细胞培养的可行性。
  • Conclusion this is a serum - free culture system for developing a conjunctival epithelial explants with improved purify and proliferative , which are important for the basic research and tissue engineer of conjunctival epithelial cells
    结论无血清培养系统的组织块培养法可为结膜上皮细胞的疾病、药理毒理研究及培养移植的结膜上皮细胞提供更为安全有效的细胞模型。
  • They used this method to improve the surface lubricity , antithrombogenicity , antibacterial , nettability and cell growth activity of the biomaterials , get a series of photo - chemical immobilized biomaterials which had good biocompatibility
    接着在这一新材料表面对鼠成纤维细胞( sto )进行无血清培养,培养2小时后,在低于lcst的温度下对sto细胞进行选择性脱附。
  • Results the epithelial cells cultured in serum - free medium not only demonstrated a significant increase in proliferative , colony - forming efficiency and cell generations , but also have the normal structural and functional properties than those in serum - containing medium
    结果无血清培养系统中结膜上皮细胞的增殖、克隆形成及细胞传代能力均较含血清培养系统高,而且具备结膜上皮细胞全部组织结构及功能特点。
  • Methods : in cultured lung explants without serum , the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity , nitric oxide ( no ) content , superoxide dismutase ( sod ) level , malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods . results : 1 . influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine , glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry , glu induced the changes of the ultrastruture of at ii iv cells
    方法:采用成年大鼠肺组织无血清培养,运用[ ~ 3h ] -胆碱掺入法测定ps主要脂质磷脂酰胆碱( pc )合成量; rt - pcr扩增检测肺组织中pc合成限速酶磷酸胆碱二胞苷酰基转移酶( cct ) mrna含量;透射电子显微镜法观察肺泡型上皮细胞和ps系统超微结构的变化;免疫组织化学染色检测glu的受体nmdar1亚单位的表达;生化测定肺组织乳酸脱氢酶( ldh )释放量和肺组织匀浆中一氧化氮合酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化酶( sod )水平以及丙二醛( mda )含量。
  • 34 we further investigate the apoptosis related gene . using different concentration of il - 16 treat jurkat cells in 1640 without serum , the expression level of bid and c - myc increase together with the increase il - 16 concentration . these suggest that bid and c - myc may take a part in the apoptosis induced by high concentration of il - 16
    结果表明,在无血清培养jurkat细胞24hr后,随着rhil ? 16浓度的增加, jurkat细胞的凋亡程度也增加,与之相应, bid和c一myc的表达水平也增加,这表示bdc m厂可能参与il叫6诱导的扣水ct细胞凋亡的调节。
  • A successful cell strain for biopharmaceutical production must conform to the following characteristics : the cells could produce high level of recombinant proteins ; the cell should be adapted to serum - free or protein - free medium ; the cells should be resistant to adverse culture conditions , which means the cells should have some anti - apoptosis property ; if not cultured i n suspension , the cells should also be able to grow in adherence
    一株成功的工程细胞除了要求目的蛋白的表达量高外,还必须适应无血清培养基培养;必须具有对不利环境的抵抗能力,即抗凋亡能力;对于非直接悬浮培养的细胞,还必须具备在无血清培养条件下的贴壁能力。
  • Using the mouse fetal ovary serum - free culture model , fetal ovaries from 14 day post coitus ( 14 dpc ) mouse were cultured , and treated by ay9944 - a - 7 , nystatin and rs - 21745 . the results showed that 0 . 025 , 0 . 0625 and 0 . 125 um ay9944 - a - 7 or 25 , 50 and 75 iu / ml nystatin increased the total number of follicles per ovary significantly ; however , ay9944 - a - 7 and nystatin at the same doses could n ' t cause the same effect on the number of growing follicles and the average diameter of five largest follicles per ovary . 50 u . m rs - 21745 decreased the total number of follicles , the number of growing follicles and diameter of follicles per ovary significantly after 48 h
    首先利用小鼠胚胎卵巢的体外无血清培养模型,培养妊娠14天( 14daypost - coitus , 14dpc )小鼠胚胎卵巢,分别添加能促进mas积累的ay9944 ,制霉菌素,和能抑制mas产生的rs - 21745进行处理,结果表明: 0 . 025 、 0 . 0625利0 . 125 m的ay9944 - a - 7与25 、 50和75iu ml的制霉菌素能显著提高卵巢中形成卵泡的总数量,但是对生长卵泡数和卵泡直径的作用不同;而mas合成抑制剂rs - 21745能够显著降低形成卵泡的总数量。
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